Mouse Anti-FGFR4 Recombinant Antibody (clone LD1) (CAT#: NS-028CN)

Figure 1 LD1 binds to human (h), mouse (m), and cynomolgus monkey (c) FGFR4, but does not bind to hFGFR1, hFGFR2, or hFGFR3.

The binding of LD1 to immobilized FGFR-Fc chimeric proteins was determined by solid phase binding assay.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 2 Binding of LD1 to hFGFR4 expressed at the cell surface of stably transfected HEK293 cells as measured by FACS (RFU  =  Relative Fluorescence Unit).

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 3 The binding of LD1 to immobilized hFGFR4-Flag chimeric proteins bearing point mutations as measured by a solid phase binding assay.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 4 The binding of LD1 to hFGFR4-Flag chimeric proteins bearing point mutations as evaluated by Western blot.

Mutated proteins were electrophoresed and sequentially immunoblotted using LD1, an anti-FGFR4 (8G11), and an anti-Flag antibody.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 5 A, LD1 inhibits FGFR4 binding to FGF1 and FGF19 as determined by solid phase binding assay. B, LD1 inhibits FGF1-stimulated proliferation of BaF3 cells stably expressing FGFR4/R1. C, LD1 inhibits FGFR4 signaling in L6 cells stably expressing FGFR4. D, Cell surface expression of FGFR4 protein in a subset of liver tumor cell lines as determined by FACS analysis using LD1.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 6 A, LD1 inhibits FGFR4 signaling in HEP3B cells as evaluated by Western blot. B, LD1 inhibits the FGFR4-regulated CYP7A1 repression in HEP3B cells. CYP7A1 levels are represented as fold expression relative to the level in untreated cells. C, LD1 inhibits FGFR4-regulated FOS expression in a panel of liver cancer cell lines. The results are represented as fold expression relative to the FOS level in untreated cells. D, Inhibition of colony formation by repression of FGFR4 expression in JHH5 cells stably transfected with an FGFR4 shRNA doxycycline-inducible vector. E, Enumeration of LD1-inhibited liver cancer cell line colony formation. The values are represented as percent of the number of colonies enumerated in the absence of added LD1. F, LD1 inhibits HCC cell line colony formation.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).