Anti-HCMV gH Recombinant Antibody (3G16) (CAT#: MRO-1045LC)

Figure 1 HCMV gH/gL antibody binding competition.

HCMV gH/gL antibody binding competition in an ELISA assay. On the x-axis are the individual capturing monoclonal antibodies; on the y-axis is the optical density determined spectrophotometrically at 450 nm. Each graph represents the results from addition of one biotinylated detection antibody. Significant reduction in signal indicates competition between the capturing antibody and the detection antibody.

Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human Antibodies

Figure 2 Single-particle EM of the gH/UL116 complex.

Purified gH/UL116/3G16 ternary complexes were analyzed by single-particle electron microscopy.

The Human Cytomegalovirus UL116 Gene Encodes an Envelope Glycoprotein Forming a Complex with gH Independently from gL

Figure 3 VR1814-MSL109R Has Escaped MSL-109.

VR1814 or VR1814-MSL109R was incubated with 10-fold dilutions of antibody for 1 hr prior to the infection of NN-NHDF cells. Twenty-four hours p.i., cells were stained for IE1/2 protein. The IC₅₀ value was calculated for each antibody using the percentage of IE1/2-positive cells compared to the untreated control.

Human Cytomegalovirus Escapes a Naturally Occurring Neutralizing Antibody by Incorporating It into Assembling Virions