Rabbit Anti-STAT1 Polyclonal Antibody (CAT#: VS3-WK1565)

• Immunogen
• Synthesized peptide derived from human STAT1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human STAT1

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ELISA

• Conjugate
• Unconjugated

• Clonality
• Polyclonal

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Aggregation
• < 5%

• Format
• Liquid

• Concentration
• 1 mg/mL

• Buffer
• PBS, 0.5% BSA, 50% Glycerol

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:500) and ELISA (1:10000-1/20000).

• Alternative Names
• CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91

• Gene ID
• 6772

• UniProt ID
• P42224

• Long Name
• Signal transducer and activator of transcription 1

• Sequence Similarities
• Belongs to the transcription factor STAT family.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Phosphorylated on tyrosine and serine residues in response to a variety of cytokines/growth hormones including IFN-alpha, IFN-gamma, PDGF and EGF (PubMed:28753426, PubMed:26479788).
  Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus (PubMed:21135090).
  Upon EGF stimulation, phosphorylation on Tyr-701 (lacking in beta form) by JAK1, JAK2 or TYK2 promotes dimerization and subsequent translocation to the nucleus (PubMed:7657660, PubMed:28753426).
  Growth hormone (GH) activates STAT1 signaling only via JAK2 (PubMed:7657660).
  Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:17561467, PubMed:19088846).
  Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2, CAMK2/CAMKII and CK2 in response to IFN-gamma stimulation, is required for maximal transcriptional activity (PubMed:7543024, PubMed:15322115, PubMed:16799645, PubMed:17897103).
  Phosphorylated on Ser-727 by CAMK2/CAMKII in response to IFN-gamma stimulation and calcium mobilization, promoting activity (PubMed:11972023, PubMed:16257975).
  Phosphorylated by CAMK2/CAMKII in response to IFN-beta stimulation and calcium mobilization in epithelial cells, promoting activity (PubMed:35568036).
  Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS (PubMed:17897103).
  Phosphorylation on Ser-727 by PRKCD induces apoptosis in response to DNA-damaging agents (PubMed:15322115).
  Phosphorylated on tyrosine residues when PTK2/FAK1 is activated; most likely this is catalyzed by a SRC family kinase (PubMed:11278462).
  Dephosphorylation on tyrosine residues by PTPN2 negatively regulates interferon-mediated signaling (PubMed:12138178).
  Upon viral infection or IFN induction, phosphorylation on Ser-708 ^oCcurs much later than phosphorylation on Tyr-701 and is required for the binding of ISGF3 on the ISREs of a subset of IFN-stimulated genes IKBKE-dependent (PubMed:22065572).
  Phosphorylation at Tyr-701 and Ser-708 are mutually exclusive, phosphorylation at Ser-708 requires previous dephosphorylation of Tyr-701 (PubMed:22065572).
  Sumoylated with SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
  ISGylated.
  Mono-ADP-ribosylated at Glu-657 and Glu-705 by PARP14; ADP-ribosylation prevents phosphorylation at Tyr-701 (PubMed:27796300).
  However, the role of ADP-ribosylation in the prevention of phosphorylation has been called into question and the lack of phosphorylation may be due to sumoylation of Lys-703 (PubMed:29858569).
  Monomethylated at Lys-525 by SETD2; monomethylation is necessary for phosphorylation at Tyr-701, translocation into the nucleus and activation of the antiviral defense.
  (Microbial infection) Ubiquitinated by Herpes simplex virus 2 E3 ubiquitin ligase ICP22.

• Protein Refseq
• NP_009330.1; NP_644671.1

• Function
• Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2.

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1517-YJ
• Sheep Anti-Rabbit IgG-heavy and light chain Secondary antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody