Human Anti-HIV gp120 Recombinant Antibody (clone 21H) (CAT#: FAMAB-0093WJ)

Figure 1 Inhibition of HIV-cell binding by sCD4.

MN virus was preincubated with increasing concentrations of sCD4 for 2 h at 37C before addition of the A3.01 cells. The virus binding test was carried out as described for Fig. 1, and data are represented as percentage of binding. To measure virus infectivity, serial dilutions of a sample of the same sCD4-treated virus used for the binding test were incubated with A3.01 cells for 2 h at 37C before culture for 10 d, and the viral titer calculated at peak p24 production in the culture supernatant. Neutralization is represented as reduction in infectivity (TCID 50 ). Figure 4. Inhibition of HIV-1 binding and infectivity by anti-gp120CD4bs-specific mAbs.

Ugolini, S., Mondor, I., Parren, P. W., Burton, D. R., Tilley, S. A., Klasse, P. J., & Sattentau, Q. J. (1997). Inhibition of virus attachment to CD4+ target cells is a major mechanism of T cell line–adapted HIV-1 neutralization. The Journal of experimental medicine, 186(8), 1287-1298.

Figure 2 Immunoprecipitations of soluble wild-type and deleted envelope gly-coproteins.

The efficient recognition of wild-type and D82/D128-194/D298-329/DC5 glycoproteins by several broadly neutralizing, CD4 binding site anti-bodies is shown.

Wyatt, R. I. C. H. A. R. D., Desjardin, E. L. I. Z. A. B. E. T. H., Olshevsky, U., Nixon, C. H. R. I. S., Binley, J. A. M. E. S., Olshevsky, V. A. R. D. A., & Sodroski, J. O. S. E. P. H. (1997). Analysis of the interaction of the human immunodeficiency virus type 1 gp120 envelope glycoprotein with the gp41 transmembrane glycoprotein. Journal of Virology, 71(12), 9722-9731.

Figure 3 Neutralization of recombinant viruses by sCD4 and MAbs.

Recombinant viruses, carrying either HXBc2 or primary envelopes, were preincubated with the indicated concentrations of 21h for 2 h at 37C. Human PBMCs were used as target cells, and the experiments were repeated two or three times to control for variability in donor PBMCs (graphs show representative experiments).

Karlsson, G. B., Gao, F., Robinson, J., Hahn, B., & Sodroski, J. (1996). Increased envelope spike density and stability are not required for the neutralization resistance of primary human immunodeficiency viruses. Journal of virology, 70(9), 6136-6142.