Human Anti-HIV gp120 Recombinant Antibody (clone m14) (CAT#: FAMAB-0242JF)
Recombinant monoclonal antibody specially binds to the CD40 binding site of HIV gp120 and gp140. This human anti-gp120 antibody (m14) is a novel CD4bs cross-reactive HIV-1 neutralizing antibody that exhibits a novel inhibitory profile, and may have implications for our understanding of the mechanisms of immune evasion and for the development of inhibitors and vaccines.
Specific Inquiry
IHC
Figure 1 Representative images of gp120 exposed primary mixed human CNS aggregate cultures at 4 weeks in vitro after Asc-p supplementation.
Error bars: +/- 1 standard error.
Walsh, K. A., Megyesi, J. F., Wilson, J. X., Crukley, J., Laubach, V. E., & Hammond, R. R. (2004). Antioxidant protection from HIV-1 gp120-induced neuroglial toxicity. Journal of neuroinflammation, 1(1), 1-14.
IF
Figure 2 Immunofluorescence imaging by confocal microscopy was performed for detection of iNOS antigens.
Each panel of images shows individual fluorophores and merged fluorophores with colocalization represented in yellow.
Walsh, K. A., Megyesi, J. F., Wilson, J. X., Crukley, J., Laubach, V. E., & Hammond, R. R. (2004). Antioxidant protection from HIV-1 gp120-induced neuroglial toxicity. Journal of neuroinflammation, 1(1), 1-14.
WB
Figure 3 Western blot analysis of the gp120 on the patient samples.
Molecular weight standards are shown at the right.
Bettaieb, A., Oksenhendler, E., Duedari, N., & Bierling, P. (1996). Cross‐reactive antibodies between HIV‐gp120 and platelet gpIIIa (CD61) in HIV‐related immune thrombocytopenic purpura. Clinical & Experimental Immunology, 103(1), 19-23.
FC
Figure 4 Recognition of recombinant HIV-1 gp120-coated and HIV-1 infected target cells by specific anti-gp120 antibodies.
Data shown are the results of at least 3 independent experiments.
Parekh, B. S., Shaffer, N., Pau, C. P., Abrams, E., Thomas, P., Pollack, H., ... & Rogers, M. (1991). Lack of correlation between maternal antibodies to V3 loop peptides of gp120 and perinatal HIV-1 transmission. The NYC Perinatal HIV Transmission Collaborative Study. AIDS (London, England), 5(10), 1179-1184.
ELISA
Figure 5 Binding specificities of mAb is determined by ELISA.
All results are representative of at least four independent experiments.
Devico, L., Rahman, R., Welch, J., Crowley, R., Lusso, P., Sarngadharan, M. G., & Pal, R. (1995). Monoclonal antibodies raised against covalently crosslinked complexes of human immunodeficiency virus type 1 gp120 and CD4 receptor identify a novel complex-dependent epitope on gp120. Virology, 211(2), 583-588.
Specifications
- Host Species
- Human
- Derivation
- Human phage display Fab library
- Type
- Human IgG1
- Specificity
- HIV gp120, HIV gp140,
- Species Reactivity
- HIV
- Clone
- m14
- Applications
- WB, ELISA, IHC, FC, IF
- Related Disease
- HIV-1 infection
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV gp120 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry, Immunofluorescence.
Target
- Alternative Names
- Human immunodeficiency virus; HIV; Envelope glycoprotein GP120; gp120; HIV envelope
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-412 | Recombinant Anti-HIV gp120 Antibody | ELISA, WB, Dot, FuncS | IgG |
| HPAB-1452-FY | Human Anti-HIV gp120 Recombinant Antibody (HPAB-1452-FY) | Neut, ELISA, FC, Block | Human IgG |
| HPAB-1454-FY | Human Anti-HIV gp120 Recombinant Antibody (HPAB-1454-FY) | Neut, ELISA, FC, Block | Human IgG |
| FAMAB-0232JF | Human Anti-HIV gp120 Recombinant Antibody (clone 257-D) | ELISA, FuncS | Human IgG1 |
| FAMAB-0177WJ | Human Anti-HIV gp120 Recombinant Antibody (clone SF12) | ELISA, WB, Neut | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-412-F(E) | Recombinant Anti-HIV gp120 Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| MHH-412-F(E) | Recombinant Human Anti-HIV gp120 Antibody Fab Fragment | Neut, IF, FuncS | Fab |
| HPAB-1452-FY-S(P) | Human Anti-HIV gp120 Recombinant Antibody; scFv Fragment (HPAB-1452-FY-S(P)) | Neut | Human scFv |
| HPAB-1454-FY-S(P) | Human Anti-HIV gp120 Recombinant Antibody; scFv Fragment (HPAB-1454-FY-S(P)) | Neut | Human scFv |
| FAMAB-0232JF-F(E) | Human Anti-HIV gp120 Recombinant Antibody (clone 257-D); Fab Fragment | ELISA, FuncS | Human Fab |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B053(A) | Recombinant Anti-HIV gp120 Intrabody [(D-Arg)9] | ELISA, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B053(G) | Recombinant Anti-HIV gp120 Intrabody [+36 GFP] | PCA, Neut, FuncS | scFv-(+36GFP) |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-182LC | Anti-HIV gp120 Recombinant Antibody (58.2) | ELISA | Human antibody |
| MRO-183LC | Anti-HIV gp120 Recombinant Antibody (59.1) | ELISA | Human antibody |
| MRO-182LC-S(P) | Anti-HIV gp120 Recombinant Antibody scFv Fragment (58.2) | ELISA | Human antibody |
| MRO-183LC-S(P) | Anti-HIV gp120 Recombinant Antibody scFv Fragment (59.1) | ELISA | Human antibody |
| MRO-182LC-F(E) | Anti-HIV gp120 Recombinant Antibody Fab Fragment (58.2) | ELISA | Human antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0556YY-S(P) | Human Anti-HIV gp120 Recombinant Antibody (clone DRVIA7H+gDRVI01-L57); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-0555YY-S(P) | Human Anti-HIV gp120 Recombinant Antibody (clone DRVIA7H+gDRVI01-L40); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-0543YY-S(P) | Human Anti-HIV gp120 Recombinant Antibody; scFv Fragment (HPAB-0543YY-S(P)) | ELISA, FuncS | Human scFv |
| HPAB-0547YY-S(P) | Human Anti-HIV gp120 Recombinant Antibody; scFv Fragment (HPAB-0547YY-S(P)) | ELISA, FuncS | Human scFv |
| FAMAB-0290JF-S(P) | Human Anti-HIV gp120 Recombinant Antibody (clone JL427); scFv Fragment | ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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