This is a monoclonal antibody that specifically reacts with oligomeric Aβ as well as amyloid plaques in the brain. The antibody inhibits Aβ amyloid fibril formation and Aβ-mediated cytotoxicity in vitro.
Figure 1 Optimization of scFv59 expression in the brain via rAAV serotype 1, 2, and 5 and theirpossible cytotoxicity in mice
Three serotypes of rAAV (1, 2, and 5) encoding scFv59 were injected into the right lateralventricle of C57BL/6 mice and, 3 months after injection, scFv59 expressed in the brain wasvisualized by immunohistochemistry using anti-FLAG M2 antibody (scFv59 contains theFLAG sequence as a marker). (a) PBS-injected mice were used as negative controls. Nostaining is observed in PBS-injected mice. (b) Widespread, symmetrical expression ofscFv59 is seen in both sides of the neocortex and the hippocampus in mice injected withrAAV1-CAscFv59. (c) Expression of scFv59 is limited mostly to a small part of the righthippocampus in mice subjected to rAAV2-CAscFv59 injection. (d) Widespread, symmetrical expression of scFv59 in mice injected with rAAV5-CAscFv59, whichresembles the expression pattern by rAAV1-CAscFv59. Levels of scFv59 expression inrAAV1-CAscFv59-injected mice are comparable to those in rAAV5-CAscFv59-injectedmice. To assess cytotoxicity and inflammation potentially associated with AAV-mediatedbrain expression of scFv59, brain sections were subjected to TUNEL assay (e–h) andimmunohistochemistry (i–l), respectively, using anti-CD11b antibody for activatedmicroglia. Apoptotic cells identified by TUNEL are seen in the brain injected with rAAV1-CAscFv59 (f) but not in the brains injected with PBS (e), rAAV2-CAscFv59 (g), andrAAV5-CAscFv59 (h). The inset in (f) is a higher magnification of the boxed area. ScatteredCD11b-positive cells are seen in the hippocampus of mice injected with rAAV1-CAscFv59(j) but not in mice injected with PBS (i), rAAV2-CAscFv59 (k), and rAAV5-CAscFv59 (l).The inset in (j) is a higher magnification of one of CD11b-positive microglia indicated byarrow heads. Scale bars 2.0 mm (a–d) and 500 μm (e–l).
Kou, J., Kim, H., Pattanayak, A., Song, M., Lim, J. E., Taguchi, H., ... & Fukuchi, K. I. (2011). Anti-amyloid-β single-chain antibody brain delivery via AAV reduces amyloid load but may increase cerebral hemorrhages in an Alzheimer's disease mouse model. Journal of Alzheimer's Disease, 27(1), 23-38.
Figure 2 Five months after rAAV5-CAscFv59 injection, CSF (A) and hippocampal homogenates (B) were prepared and subjected to 10–20% SDS PAGE followed by western blotting using anti-FLAG M2 antibody.
(A) scFv59 is identified as an approximately 30 kDa fragment in the CSF isolated from mice subjected to rAAV5-CAscFv59 injection. Lane 1: Molecular size markers; lane 2: Ten ng of purified scFv59 as a positive control; lane 3 Blank; lane 4: Ten μl of CSF from a PBS-injected mouse; and lane 5: Ten μl of CSF from a rAAV5-CAscFv59-injected mouse. (B) scFv59 is seen as 30–35 kDa fragments in the hippocampus of mice subjected to rAAV5-CAscFv59 injection. Lane 1: Molecular size markers; lane 2: Ten ng of purified scFv59 as a positive control; lane 3: Hippocampal homogenate from a PBS-injected mouse; and lane 4 and 5: Hippocampal homogenates from rAAV5-CAscFv59-injected mice.
Kou, J., Kim, H., Pattanayak, A., Song, M., Lim, J. E., Taguchi, H., ... & Fukuchi, K. I. (2011). Anti-amyloid-β single-chain antibody brain delivery via AAV reduces amyloid load but may increase cerebral hemorrhages in an Alzheimer's disease mouse model. Journal of Alzheimer's Disease, 27(1), 23-38.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-25 | Recombinant Anti-human Aβ42 Antibody | WB, FC, FuncS | IgG |
MHH-25 | Recombinant Human Anti-human Aβ42 Antibody | FC, WB, FuncS | IgG |
HPAB-0511-WJ | Mouse Anti-Aβ42 Recombinant Antibody (clone 3F5) | Inhib, ELISA, Live cell imaging, FuncS | Mouse IgG2b |
HPAB-0586-FY | Human Anti-Aβ42 Recombinant Antibody (clone M06) | DB, ELISA | Human IgG |
HPAB-2064-FY | Human Anti-Aβ42 Recombinant Antibody (clone 8F5) | Neut, ELISA, DB, IF | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-25-F(E) | Recombinant Anti-human Aβ42 Antibody Fab Fragment | Neut, IF, FuncS | Fab |
MHH-25-F(E) | Recombinant Human Anti-human Aβ42 Antibody Fab Fragment | IF, FC, Biosensors, FuncS | Fab |
HPAB-0511-WJ-F(E) | Mouse Anti-Aβ42 Recombinant Antibody (clone 3F5); Fab Fragment | ELISA, FC | Mouse Fab |
HPAB-0586-FY-F(E) | Human Anti-Aβ42 Recombinant Antibody (clone M06); Fab Fragment | DB, ELISA | Human Fab |
HPAB-2064-FY-F(E) | Human Anti-Aβ42 Recombinant Antibody (clone 8F5); Fab Fragment | Neut, ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-25-S(P) | Recombinant Anti-human Aβ42 Antibody scFv Fragment | WB, FC, FuncS | scFv |
MHH-25-S(P) | Recombinant Human Anti-human Aβ42 Antibody scFv Fragment | Neut, WB, FuncS | scFv |
FAMAB-0160-CN-S(P) | Human Anti-Aβ42 Recombinant Antibody (clone scFv59); scFv Fragment | WB, ELISA, IF, IHC | Human scFv |
HPAB-0511-WJ-S(P) | Mouse Anti-Aβ42 Recombinant Antibody (clone 3F5); scFv Fragment | ELISA, FC | Mouse scFv |
HPAB-0586-FY-S(P) | Human Anti-Aβ42 Recombinant Antibody (clone M06); scFv Fragment | DB, ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B042(A) | Recombinant Anti-human Aβ42 Intrabody [(D-Arg)9] | CO-IP, FuncS | scFv-(D-Arg)9 |
IAB-B042(G) | Recombinant Anti-human Aβ42 Intrabody [+36 GFP] | ELISA, IF, Neut, FuncS | scFv-(+36GFP) |
IAB-B042(T) | Recombinant Anti-human Aβ42 Intrabody [Tat] | ELISA, Neut, FuncS | scFv-Tat |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-2023-FY-VHH | Camelid Anti-Aβ42 Recombinant Single Domain Antibody (clone VHH07) | ELISA | Llama VHH |
HPAB-2024-FY-VHH | Camelid Anti-Aβ42 Recombinant Single Domain Antibody (clone VHH05) | ELISA | Llama VHH |
HPAB-2025-FY-VHH | Camelid Anti-Aβ42 Recombinant Single Domain Antibody (clone VHH03) | ELISA | Llama VHH |
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