Mouse Anti-Aβ42 Recombinant Antibody (clone 3F5) (CAT#: HPAB-0511-WJ)
This product is a mouse antibody specific for the peptide Aβ42 N-terminal epitope, with Aβ42 peptide binding and strong, and can significantly promote phagocytosis of microglia Aβ42 peptide was dissolved and clear Aβ deposition.
Specific Inquiry
Inhib
Figure 1 3F5 inhibits the neuronal cell apoptosis induced by Aβ1–42 fibrils.
The percentage of cell apoptosis on SH-SY5Y cells stained with AnnexinV-FITC/PI. The experiments were repeated three times.
Xing, H. Y., Li, B., Peng, D., Wang, C. Y., Wang, G. Y., Li, P., ... & Chen, J. H. (2017). A novel monoclonal antibody against the N-terminus of Aβ1-42 reduces plaques and improves cognition in a mouse model of Alzheimer’s disease. PloS one, 12(6), e0180076.
ELISA
Figure 2 3F5 specifically binds the N-terminus of Aβ1–42.
Aβ1–42 (0–1 μg/mL) without FITC conjugation was preincubated with 3F5 overnight at 4˚C. Thereafter, Aβ1–42 (0–1 μg/mL) without FITC conjugation and Aβ1–42 coated on ELISA plates competitively bound with 3F5 for 90 min at 37˚C. The competitive binding of 3F5 with free or coated Aβ1–42 was detected by ELISA.
Xing, H. Y., Li, B., Peng, D., Wang, C. Y., Wang, G. Y., Li, P., ... & Chen, J. H. (2017). A novel monoclonal antibody against the N-terminus of Aβ1-42 reduces plaques and improves cognition in a mouse model of Alzheimer’s disease. PloS one, 12(6), e0180076.
Live cell imaging
Figure 3 SH-SY5Y cell images were shown by microscopy (100×).
Xing, H. Y., Li, B., Peng, D., Wang, C. Y., Wang, G. Y., Li, P., ... & Chen, J. H. (2017). A novel monoclonal antibody against the N-terminus of Aβ1-42 reduces plaques and improves cognition in a mouse model of Alzheimer’s disease. PloS one, 12(6), e0180076.
FuncS
Figure 4 3F5 reduces the neurotoxicity of Aβ1–42 fibrils in vitro.
Xing, H. Y., Li, B., Peng, D., Wang, C. Y., Wang, G. Y., Li, P., ... & Chen, J. H. (2017). A novel monoclonal antibody against the N-terminus of Aβ1-42 reduces plaques and improves cognition in a mouse model of Alzheimer’s disease. PloS one, 12(6), e0180076.
Specifications
- Immunogen
- Full-length Αβ 42
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Senile dementia Aβ42 peptide
- Species Reactivity
- Human
- Clone
- 3F5
- Applications
- Inhib, ELISA, Live cell imaging, FuncS
- Related Disease
- Senile dementia
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Inhibition, Enzyme-linked Immunosorbent Assay, Live cell imaging and Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Beta-amyloid peptide; beta-APP; Amyloid-beta; Aβ; Abeta; Abeta42
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-25 | Recombinant Anti-human Aβ42 Antibody | WB, FC, FuncS | IgG |
| MHH-25 | Recombinant Human Anti-human Aβ42 Antibody | FC, WB, FuncS | IgG |
| HPAB-0586-FY | Human Anti-Aβ42 Recombinant Antibody (clone M06) | DB, ELISA | Human IgG |
| HPAB-2064-FY | Human Anti-Aβ42 Recombinant Antibody (clone 8F5) | Neut, ELISA, DB, IF | Human IgG |
| HPAB-A1231-YJ | Human Anti-Aβ42 Recombinant Antibody (clone HT7) | ELISA, DB, Inhib | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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