Anti-Aβ42 Intrabody [Tat] (IAB-B042(T))
Recombinant anti-Aβ42 intrabody in fusion with HIV Tat works within cells and is directed against intracellur Aβ42, expressed in E. coli cells. The intrabodies, one with an endoplasmic reticulum (ER) targeting signal and one devoid of a targeting sequence, were assessed in cells harboring a doxycycline (Dox)-regulated mutant human amyloid precursor protein Swedish mutant (hAPPswe) transcription unit for their abilities to prevent Aβ peptide egress.
Specifications
- Immunogen
- human Amyloid β Peptide 42
- Host
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Can be useful in applications such as: Enzyme-linked Immunosorbent Assay; Neutralization; Functional studies
Target
- Alternative Names
- Aβ42; Amyloid β Peptide 42
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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