Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 102) (CAT#: MRO-3557CQ)
Recombinant mouse antibody to gp120. A genetically engineered single chain antibody (scFv102) was produced from neutralizing MAb 102 cDNA, covering coding variable regions of its heavy and light chains.
Specific Inquiry
WB
Figure 1 The strip immunoblot assay of mAb 102.
The same amounts of protein were incubated with each strip. Both antibodies exhibited the same binding specificity in the immunoblot assays.
Lane 1: Molecular marker of HIV-1 antigens, Lane 2: mAb 102 and Lane 3: scFv 102.
IF
Figure 2 Indirect immuno-fluorescence assays obtained by staining Hela-Env cells with antibodies.
The Hela-Env cells were stained with mAb 102 (Left), negative control antibody (Middle) and scFv 102 (Right), respectively.
ELISA
Figure 3 Indirect sandwich ELISA enzyme immunoassay of product VS-0323-CJ25.
The binding affinities of the scFv 102 and its parent mAb were measured by further capture of the antigen using HIV-1 positive serum and anti-human IgG.
Neut
Figure 4 The neutralization assay of product VS-0323-CJ25 and its scFv.
The neutralization ability to Inhibit the infection of human MT4 T-lymphoid cells was performed using a primary isolate of HIV-1.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Recognizes HIV-1 gp120
- Species Reactivity
- HIV-1
- Clone
- 102
- Applications
- Inhibition, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Neutralization, Immunoassay
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- MRO-3557CQ had high specificity to HIV-1 gp120 showed in immuno-blotting assays and immuno-fluorescence (IF) detection, and ELISA analysis. In neutralization assays, MRO-3557CQ exhibited efficient interference with viral replication and inhibition of viral infection.
ELISA: 20-1000 ng/mL
Neutralization: 1 nM-1000 nM
Target
- Alternative Names
- Human immunodeficiency virus type 1; HIV-1; Envelope glycoprotein GP120; gp120; HIV envelope
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 102"
See other products for "HIV-1 gp120"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135) | ELISA, Neut | Human IgG |
| PABL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d) | Neut, WB, ELISA, FuncS | Human IgG |
| PABL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-156 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC-CH31) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-160 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 59.1) | WB, ELISA, FuncS | Mouse IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-160 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone F105); scFv Fragment | FC, Neut | Human scFv |
| PSBW-178 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone b12); scFv Fragment | Neut, ELISA | Human scFv |
| MRO-3081CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH276); Fab Fragment | Neut | Human Fab |
| MRO-3082CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH374); Fab Fragment | Neut | Human Fab |
| MRO-3083CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH375); Fab Fragment | Neut | Human Fab |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91C2) | ELISA | Single domain antibody |
| MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91F10) | ELISA | Single domain antibody |
| MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91E1) | ELISA | Single domain antibody |
| MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91B5) | ELISA | Single domain antibody |
| MRO-032LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91H9) | ELISA | Single domain antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-041LC | Anti-HIV-1 gp120 Recombinant Antibody (D59/A2) | ELISA | Mouse antibody |
| MRO-042LC | Anti-HIV-1 gp120 Recombinant Antibody (F58/H3) | WB, ELISA | Mouse antibody |
| MRO-043LC | Anti-HIV-1 gp120 Recombinant Antibody (P1/D12) | ELISA | Mouse antibody |
| MRO-044LC | Anti-HIV-1 gp120 Recombinant Antibody (P4/D10) | ELISA | Mouse antibody |
| MRO-045LC | Anti-HIV-1 gp120 Recombinant Antibody (A47/B1) | ELISA | Mouse antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-125LC | Anti-HIV-1 gp120 Recombinant Antibody (N6) | Neut, ELISA | Human antibody |
| MRO-130LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-130LC) | ELISA, Neut, SPR | Human IgG |
| MRO-136LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900973) | ELISA | Human antibody |
| MRO-137LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900972) | ELISA | Human antibody |
| MRO-138LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900990) | ELISA | Human antibody |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
| MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
| MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-3585CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone T8); scFv Fragment | ELISA, WB | Mouse scFv |
| MRO-3586CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone V3-G2-10); scFv Fragment | Neut | Mouse scFv |
| MRO-3587CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone V3-G2-25); scFv Fragment | Neut | Mouse scFv |
| MRO-3588CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone V3-W1-2); scFv Fragment | Neut | Mouse scFv |
| MRO-3589CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone V3-W1-8); scFv Fragment | Neut | Mouse scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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