Rat Anti-NOTCH1 Recombinant Antibody (clone bTAN 20) (CAT#: HPAB-0085-YJ)

Figure 1 Intracellular Notch Epitopes Are Carried by a 110 kDa Fragment

Western blot analysis of human cell lines, human tissues, Drosophila cell lines, rat and Drosophila embryos. The cell source of each lysate is indicated above the lanes. Notch1 with antibody bTAN 20.

Blaumueller, C. M., Qi, H., Zagouras, P., & Artavanis-Tsakonas, S. (1997). Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane. Cell, 90(2), 281-291.

Figure 2 Whole cell lysates of Jurkat cells transiently transfected with GFP (first lane) or 5
g of AcN1GFP (second lane) were analyzed for the expression of full-length Notch-1 protein using the bTan20 antibody.

Sade, H., Krishna, S., & Sarin, A. (2004). The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells. Journal of Biological Chemistry, 279(4), 2937-2944.

Figure 3 Large pleomorphic cells with prominent nucleoli, ALCL tumor cells, are intensely labeled by anti-Notch1 antibody.

Jundt, F., Anagnostopoulos, I., Förster, R., Mathas, S., Stein, H., & Dörken, B. (2002). Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma. Blood, 99(9), 3398-3403.