Recombinant Rabbit Anti-NOTCH1 Antibody (clone R02-2G9)
CAT#: VS3-FY1049
This product is a recombinant rabbit antibody that recognizes NOTCH1. This antibody has been reported for use in Western Blot. The clone R02-2G9 is specific for human, rat NOTCH1. The antigen is human notch1 synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67168/1315_F7_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22466/61564_A_3_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Fibroblasts Staining: High Intensity: Strong Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peripheral nerve/ganglion Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8112/61559_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HaCaT, HDLM-2, HMC-1, MOLT-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000148400-NOTCH1
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❯
WB
Figure 1. Recombinant Rabbit Anti-NOTCH1 Antibody (clone R02-2G9) in WB.
Western Blot analysis of Notch1 in C6, Hela lysates using Notch1 Antibody.
❮
❯
Specifications
- Immunogen
- Human Notch1 synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat NOTCH1
- Species Reactivity
- Human, Rat
- Clone
- R02-2G9
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 273 kDa; Observed MW: 120 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Notch 1; Translocation-Associated Notch Protein TAN-1; TAN1; HN1; Notch (Drosophila) Homolog 1 (Translocation-Associated); Notch Homolog 1, Translocation-Associated (Drosophila); Notch Homolog 1, Translocation-Associated
- Gene ID
- 4851
- UniProt ID
- P46531
- Sequence Similarities
- Belongs to the NOTCH family.
- Cellular Localization
- Cell membrane, Membrane, Nucleus
- Post Translation Modifications
- Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form (By similarity).
Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT) (PubMed:24226769).
Following endocytosis, this fragment is then cleaved by one of the catalytic subunits of gamma-secretase (PSEN1 or PSEN2), to release a Notch-derived peptide containing the intracellular domain (NICD) from the membrane (PubMed:30598546).
Phosphorylated.
O-glycosylated on the EGF-like domains (PubMed:24226769).
O-glucosylated at Ser-435 by KDELC1 and KDELC2 (PubMed:30127001).
Contains both O-linked fucose and O-linked glucose in the EGF-like domains 11, 12 and 13, which are interacting with the residues on DLL4 (By similarity).
O-linked glycosylation by GALNT11 is involved in determination of left/right symmetry: glycosylation promotes activation of NOTCH1, possibly by promoting cleavage by ADAM17, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO) (PubMed:24226769).
MFNG-, RFNG- and LFNG-mediated modification of O-fucose residues at specific EGF-like domains results in inhibition of its activation by JAG1 and enhancement of its activation by DLL1 via an increased binding to DLL1 (By similarity).
Ubiquitinated. Undergoes 'Lys-29'-linked polyubiquitination by ITCH; promotes the lysosomal degradation of non-activated internalized NOTCH1 (PubMed:18628966, PubMed:23886940).
Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch (PubMed:24226769).
Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity).
Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD (By similarity).
- Protein Refseq
- NP_060087.3
- Function
- Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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- Product Name
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- CAT
- Product Name
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| TAB-539MZ-F(E) | Rat Anti-NOTCH1 Recombinant Antibody; Fab Fragment (TAB-539MZ-F(E)) | WB, Competition ELISA | Rat Fab |
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| CAT | Product Name | Application | Type |
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| TAB-541MZ-S(P) | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-541MZ-S(P)) | WB, Competition ELISA | Humanized scFv |
| TAB-542MZ-S(P) | Human Anti-NOTCH1 Recombinant Antibody; scFv Fragment (TAB-542MZ-S(P)) | WB, Competition ELISA | Humanized scFv |
| TAB-541MZ-F(E) | Human Anti-NOTCH1 Recombinant Antibody; Fab Fragment (TAB-541MZ-F(E)) | WB, Competition ELISA | Humanized Fab |
| TAB-542MZ-F(E) | Human Anti-NOTCH1 Recombinant Antibody; Fab Fragment (TAB-542MZ-F(E)) | WB, Competition ELISA | Humanized Fab |
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| CAT | Product Name | Application | Type |
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| HPAB-AP753-YC-F(E) | Human Anti-NOTCH1 Recombinant Antibody (clone N1wc104); Fab Fragment | ELISA, FC | Human Fab |
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|---|---|---|---|
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| NS-061CN | Mouse Anti-NOTCH1 Recombinant Antibody (clone 2G10) | FC, IF, ELISA | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
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|---|---|---|---|
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| HPAB-AP751-YC | Human Anti-NOTCH1 Recombinant Antibody (clone hN1wc155) | Block | Human IgG1 |
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| VS-0924-YC79 | Rabbit Anti-NOTCH1 Antibody (VS-0924-YC79) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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