Human Anti-HIV-1 gp120 Recombinant Antibody (clone P20) (CAT#: FAMAB-0058WJ)

This product is a human monoclonal antibody targeting Human Immunodeficiency Virus Type 1 gp120. This antibody is derived phage display libraries.


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ELISA

Figure 1 Binding of P20 to the HIV-1 gp41 6-HB core.

Figure 1 Binding of P20 to the HIV-1 gp41 6-HB core.

Binding of P20 to the N peptide N36, the C peptide C34, and the 6-HB 3NC, as determined by direct ELISA.

Zhu, Y., Lu, L., Xu, L., Yang, H., Jiang, S., & Chen, Y. H. (2010). Identification of a gp41 core-binding molecule with homologous sequence of human TNNI3K-like protein as a novel human immunodeficiency virus type 1 entry inhibitor. Journal of virology, 84(18), 9359-9368.

ELISA

Figure 2 Effect of P20 on the gp41 6-HB formation between N36 and C34 by ELISA.

Figure 2 Effect of P20 on the gp41 6-HB formation between N36 and C34 by ELISA.

ADS-J1 was used as a positive control. After native polyacrylamide gel electrophoresis, the gel was visualized using a transillumination UV light source to reveal the bands containing C34-FITC, including C34-FITC alone (line 2), N36/C34-FITC complex (line 3), and N36/C34-FITC/P20 complex (line 5).

Zhu, Y., Lu, L., Xu, L., Yang, H., Jiang, S., & Chen, Y. H. (2010). Identification of a gp41 core-binding molecule with homologous sequence of human TNNI3K-like protein as a novel human immunodeficiency virus type 1 entry inhibitor. Journal of virology, 84(18), 9359-9368.

Inhib

Figure 3 Effect of P20 on HIV-1 Env-mediated membrane fusion.

Figure 3 Effect of P20 on HIV-1 Env-mediated membrane fusion.

Inhibition of P20 on HIV-1 Env-mediated syncytium formation. GST protein was used as the negative control.

Zhu, Y., Lu, L., Xu, L., Yang, H., Jiang, S., & Chen, Y. H. (2010). Identification of a gp41 core-binding molecule with homologous sequence of human TNNI3K-like protein as a novel human immunodeficiency virus type 1 entry inhibitor. Journal of virology, 84(18), 9359-9368.


Specifications

  • Host Species
  • Human
  • Derivation
  • Phage display library
  • Type
  • Human IgG
  • Specificity
  • HIV-1 gp120
  • Species Reactivity
  • HIV
  • Clone
  • P20
  • Applications
  • ELISA, Neut
  • Related Disease
  • HIV infection

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The HIV-1 gp120 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Neutralization.

Target

  • Alternative Names
  • Human immunodeficiency virus Envelope glycoprotein GP120; HIV gp120

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

Recombinant Antibody

Chimeric Antibody

CAT Product Name Application Type
MRO-135LC Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) ELISA, WB, Neut Chimeric antibody (mouse/human)
MRO-135LC-S(P) Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)
MRO-135LC-F(E) Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4220 Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) WB, ELISA Rabbit IgG

Humanized Antibody

CAT Product Name Application Type
PABX-101-S(P) Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment WB, ELISA, FuncS Human scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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