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10-FnIII anti-NFKBIA (SFN-L017)

The tenth fibronectin type III domain (10Fn3) that bound phospho-IkappaBalpha peptide was isolated from the library using mRNA Display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=18 nM) for phospho-IkappaBalpha peptide as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.      
Datasheet
Specifications
Scaffold Name
10-FnIII
Origin
Fibronectin
Core Structure
β-sandwich of 7 β-strands
Variable Regions
2–3 Loops
Target
Phospho-IkappaBalpha peptide
Species Reactivity
Human
Expression Host
E. coli
Affinity Constant
18 nM
Applications
ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
Alternative Names
Fibronectin; 10-FnIII; IKBA; MAD-3; NFKBI
Entrez Gene ID
4792
UniProt ID
P25963

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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