Custom Antibody-coupled Liposome Development Services

Creative Biolabs offers a full suite of services that include formulation optimization, characterization, stability studies, and regulatory support for the production of various antibody-coupled liposomes. Our antibody-coupled liposome platforms guarantee stable encapsulation and precise delivery of antibodies, maximizing their therapeutic efficacy while reducing off-target effects.

Platform

Liposome drug delivery technology has achieved significant advancements in the realm of biomedicine. Numerous research studies have substantiated the advantages of liposomes in drug component loading, overcoming cellular and tissue uptake barriers, and enhancing drug distribution at targeted sites. The co-encapsulation of multiple drugs within liposomes allows for a synergistic mechanism among various compounds to achieve enhanced therapeutic outcomes.

At Creative Biolabs, antibody-coupled liposome platforms represent a cutting-edge strategy in drug delivery systems, especially for targeted therapies. Our lipid-based vesicles encapsulate therapeutic agents—such as drugs, proteins, or nucleic acids—and are engineered with antibodies that specifically bind to receptors on target cells.

Fig.1 Main Types of Lipid Nanoparticles.^1,3

Workflows

Currently, our antibody-coupled liposomes are a versatile tool in biomedical research and therapeutics, particularly in targeted drug delivery, imaging, and vaccine development. The workflow for constructing and utilizing antibody-coupled liposome platforms generally includes several key steps:

1. Design and Formulation of Liposomes

Selection of Lipid Composition: Choose appropriate lipids (e.g., phospholipids, cholesterol) based on the desired properties (size, stability, release profile).

Liposome Preparation: Utilize methods such as thin-film hydration, extrusion, or sonication to form liposomes. Characterization techniques (e.g., dynamic light scattering) can assess the size and polydispersity.

Sonication or Extrusion (Optional): To achieve uniform liposome size, sonicate the liposome suspension or use extrusion through polycarbonate membranes for size reduction.

2. Antibody Selection

Target Identification: Identify a suitable target antigen that is overexpressed on the surface of the target cells, such as cancer cells.

Antibody Sourcing: Acquire antibodies through hybridoma technology, recombinant DNA technology, or commercial suppliers.

Antibody Activation (if required): Depending on the specific antibody and coupling method used, activation may be necessary (for example, through NHS or EDC) to enhance the conjugation reaction.

3. Conjugation of Antibodies to Liposomes

Surface Activation of Liposomes: Modify the liposomal surfaces with functional groups (e.g., maleimide, amine) to promote conjugation.

Conjugation Reaction: Combine activated liposomes with antibodies under controlled conditions to facilitate covalent bond formation, optimizing parameters such as pH, temperature, and reaction time for maximum efficiency.

Purification: Eliminate unbound antibodies using methods like size exclusion chromatography, ultrafiltration, or dialysis.

4. Characterization of Antibody-coupled Liposomes

Size and Zeta Potential Analysis: Measure the size and surface charge of the antibody-liposome conjugates.

Binding Affinity Evaluation: Confirm that the antibodies retain their binding capabilities using assays such as ELISA or surface plasmon resonance.

Stability Assessment: Test the stability of the antibody-coupled liposomes under various storage conditions, including fluctuations in temperature.

Fig.2 Antibody-coupled Liposomes For Drug Targeting And Delivery.^1,3

Features

In our labs, antibody-coupled liposomes have become advanced drug delivery systems that utilize liposomes-tiny spherical vesicles composed of lipid bilayers-functionalized with antibodies. Our innovative technology offers numerous advantages:

• Tissue/Organ Targeted Delivery: Immunoliposomes can specifically bind to target cells or tissues that express the corresponding antigen, enhancing the concentration of the therapeutic agent at the desired site while reducing exposure to non-target tissues.
• Enhanced Efficacy: By delivering drugs directly to target cells, immunoliposomes improve the therapeutic efficacy of the payload, as elevated local concentrations are associated with better treatment outcomes.
• Reduced Side Effects: Targeted delivery minimizes systemic toxicity and side effects commonly associated with traditional drug administration, as it decreases healthy tissue exposure to the therapeutic agent.
• Versatile Payloads: Immunoliposomes can accommodate a diverse range of therapeutic agents, including small molecules, proteins, peptides, and nucleic acids, making them flexible tools in drug delivery.
• Improved Pharmacokinetics: The attachment of antibodies enhances the pharmacokinetic properties of the encapsulated drug, potentially resulting in prolonged circulation times and increased bioavailability.
• Stimulated Immune Response: In certain cases, antibody-coupled liposomes can provoke an immune response against cancer cells or pathogens, further enhancing therapeutic efficacy.
• Multifunctionality: Immunoliposomes can be engineered to carry multiple therapeutic or imaging agents concurrently, facilitating combination therapies or enabling simultaneous diagnosis and treatment (theranostics).
• Controlled Release: Liposomal formulations can be tailored for controlled or sustained release of their payloads, providing a prolonged therapeutic effect.
• Ease of Modification: The liposomal structure allows for various modifications, such as surface modification, composition alteration, and nanoparticle hybridization.
• Reduced Immunogenicity: Thoughtfully designed antibody-coupled liposomes can evade the immune system, minimizing the chances of triggering an immune response that might neutralize the therapeutic agent before it reaches its target.

Creative Biolabs concentrates on the innovative integration of liposomes and antibodies to develop potent conjugates capable of delivering drugs directly to targeted cells. We prioritize safety and efficacy by conducting comprehensive testing to ensure the performance of our conjugates before they reach you. Feel free to contact us for inquiries or to place an order.

For research use only. Not intended for any clinical use.