Antibody Fragment Production and QC Services

To assist research and pharmaceutical organizations in rapidly achieving efficient and reliable expression and purification of antibody fragments, Creative Biolabs offers a comprehensive range of advanced services, focusing on six distinct types of antibody fragments: Fab, scFv, Hexamer, BCR, TCR, and ScFv-Fc.

Introduction

Nowadays, Creative Biolabs has developed an in vitro recombinant expression platform centered around mammalian cells, offering clients an array of high-quality services. Our solutions include sequence analysis, linker design, fusion tag design, high-expression vector construction, optimization of antibody fragments expression strategies, refinement of recombinant expression conditions, and antibody fragments purification.

Clients can supply antibody fragments according to their specific needs. Creative Biolabs possesses high-yield pCBT series vectors, along with 293 and CHO cell lines, enabling the efficient secretion and expression of various forms of antibody fragments. We use advanced technology and streamlined production processes to significantly enhance the yield and purity of antibody fragments, while also reducing production time. All products undergo rigorous testing with advanced equipment such as HPLC and Mass Spectrometry (MS) to ensure they meet industry standards.

Fig.1 Antibody Fragments FAormats.^1,3

Services

In the past few years, the technology surrounding antibody fragments has gained significant attention due to its unique biological properties and potential applications in targeted therapies. Creative Biolabs is poised to offer clients comprehensive and expert preclinical contract research organization (CRO) services, with a focus on solutions for antibody fragment production. Our offerings encompass six distinct types of antibody fragments, each providing unique characteristics and applications, which we will explore in detail below:

• Fab Antibody Fragment Production & QC

Fab antibody fragments consist of the variable region of an antibody along with one light chain, retaining the advantages of high specificity and affinity. Our Fab fragment expression and purification services employ advanced yeast or cell expression systems to optimize both yield and quality. Additionally, we offer tailored purification processes to ensure the generation of highly pure Fab fragments, suitable for subsequent pharmacological and clinical studies. The application of Fab fragments is becoming increasingly widespread in neutralizing antibodies, diagnostic agents, and targeted therapies, presenting valuable collaboration opportunities for pharmaceutical companies.

• ScFv Antibody Fragment Production & QC

ScFv are formed by linking the variable regions of antibody heavy and light chains through a peptide chain, allowing them to target antigens with a smaller molecular weight. We use efficient cell lines for scFv expression to ensure that the products are stable and fully functional. Furthermore, our purification process integrates various chromatography techniques to achieve optimal separation and purity. Our service is particularly important for clients looking to enhance their research pipelines, as scFv antibody fragments play a crucial role in applications such as in vitro diagnostics, drug screening, and ligand capture technologies.

• Hexamer Antibody Production & QC

We offer a wide range of services for the expression and purification of hexameric antibodies. These Hexamer antibodies exhibit enhanced stability and affinity, allowing them to maintain their active state in complex biological environments. Our team utilizes advanced recombinant protein production techniques to ensure the efficient generation of high-quality Hexamer antibodies. By optimizing culture media and expression systems, we deliver Hexamer antibodies with excellent biological activity, meeting the rigorous demands of drug development and fundamental research.

• BCR Production & QC

B cell receptors (BCRs) are critical immune molecules, and their study is essential for understanding immune responses and developing antibody therapeutics. Our BCR expression and purification service employs unique and effective methods to provide clients with functional BCRs. Utilizing specially designed expression systems, we ensure that BCRs are accurately folded and active in their natural state, thereby providing a reliable foundation for subsequent functional studies. Our service also includes customized expression and purification of various BCR subtypes, enabling clients to obtain specific antibodies tailored to their research needs.

• Soluble TCR Production & QC

Soluble T cell receptors (TCRs) play a vital role in cancer immunotherapy and vaccine development. We provide specialized services for the expression and purification of soluble TCRs. Through optimized expression systems and purification processes, we can efficiently produce functional TCRs, offering researchers ideal models for cell experiments and drug screening. Additionally, drawing on our extensive experience, we can deliver personalized TCR solutions that cater to specific research targets.

• ScFv-Fc Fusion Antibody Production & QC

ScFv-Fc antibodies are a specialized class of recombinant antibodies formed by the fusion of scFv with the Fc region, combining the high affinity of scFv with the effector functions of the Fc portion. Based on our proprietary expression system and optimized purification process, we can efficiently produce scFv-Fc antibodies, ensuring higher expression levels and purity. Our services guarantee not only the biological activity of the antibodies but also offer various labeling and handling options to meet diverse experimental requirements. Additionally, our team provides tailored designs of expression constructs to align with specific client needs, ensuring that the produced scFv-Fc antibodies effectively target designated antigens.

Fig.2 Evaluation of Aggregation Propensity of scFv Antibody Fragments.^2,3

Advantages

• Minimizing Non-Specific Binding: Since the Fc region of antibodies can interact with Fc receptors present on various cells, employing antibody fragments can effectively reduce the non-specific binding that arises from these interactions.
• Enhanced Binding Control: In immunoprecipitation and western blotting assays, antibody fragments provide better control over binding to protein A or protein G, thereby improving the specificity of the experiments.
• Improved Tissue Penetration: Due to their smaller size, antibody fragments can penetrate tissue sections more efficiently, enhancing the effectiveness of immunohistochemistry (IHC) staining.
• Increased Sensitivity: For certain large protein epitopes, antibody fragments may enhance the sensitivity of antigen detection in solid-phase applications.
• Elimination of Fc-Related Effects: In studies focusing on antigen-antibody interactions, using antibody fragments can remove effects associated with the Fc region, such as the influence of complement fixation, leading to more purified data.
• Simplified Structural Studies: When employing techniques such as X-ray crystallography or NMR to investigate immune recognition structural regions, antibody fragments are easier to handle, reducing the complexity of the experiments.
• Reduced Immunogenicity: Antibody fragments typically exhibit lower immunogenicity compared to full-length antibodies, which helps to minimize potential immune responses.

Creative Biolabs understands that the development and production of antibody fragments require both efficiency and expertise. To address this, we offer a comprehensive range of solutions for antibody fragment production. Our advanced gene cloning technology enables us to rapidly construct optimal expression vectors and select the appropriate expression systems, such as E. coli, yeast, insect cells, or mammalian cells, for efficient production. We invite you to contact us to explore how our expertise can support your antibody fragment production project.

For research use only. Not intended for any clinical use.