With the continuous advancements in biotechnology, the significance of B cell receptors (BCRs) in the fields of immunology research and antibody development is becoming increasingly prominent. BCRs play a crucial role in the recognition of antigens and the mediation of immune responses, making the study of their structure and function essential for the development of novel antibody drugs. In response to this demand, Creative Biolabs has discovered a series of BCR expression and purification services for clients worldwide.
BCRs are vital immune molecules that play a crucial role in the immune system. They are responsible not only for recognizing and binding antigens but also for significantly influencing the pathogenesis of various tumors, including leukemia and lymphoma. As a result, there has been a notable increase in the demand for BCR expression and purification services. Creative Biolabs offers specialized BCR expression and purification services to assist clients in accelerating their drug development processes.
In our company, our team specializes in utilizing molecular cloning techniques to construct recombinant expression vectors based on the genetic target BCR sequences, covering many subtypes such as IgM and IgG. We support a range of expression systems, including E. coli, yeast, and eukaryotic cells. Moreover, For clients requiring large-scale production, we offer both batch and continuous protein production solutions, ensuring a stable and efficient supply of BCR proteins. Our facilities are equipped with large bioreactors and automated production lines to meet diverse production scale requirements.
In terms of BCR purification, we employ various chromatography techniques, including affinity chromatography, ion exchange chromatography, and gel filtration chromatography, to achieve efficient purification of the expressed BCR proteins. Our team meticulously optimizes each chromatography method to attain the best possible purification efficiency and the highest protein purity.
Fig.1 The Structure of B Cell Receptors.1,3
Nowadays, Creative Biolabs offers comprehensive services for BCR expression and purification, aiming to provide efficient and reliable support for BCR research and accelerate the development of new therapies.
Fig.2 The Analysis of BCR Repertoires.2,3
Creative Biolabs offers BCR expression and purification services, providing clients with high-quality research materials to support their innovative studies in immunology and related fields.
1. DNA Cloning and Transfection
Our BCR expression process begins with gene cloning. We employ synthetic biology techniques to acquire the target BCR gene and insert it into appropriate expression vectors for either E. coli or mammalian cells. During the transfection phase, we utilize highly efficient reagents and methods, such as lipid-based transfection or electroporation, to achieve optimal expression levels.
2. Selecting the Right Host Cells
Choosing the appropriate host cells is crucial for the expression of BCR. Depending on the characteristics of the target protein, we selectively employ various host cells, including E. coli, yeast, HEK293, and CHO cells. For BCRs that require glycosylation, Creative Biolabs primarily uses CHO cells to achieve protein modifications that closely resemble physiological conditions.
3. Optimizing Protein Expression
During the expression stage, our staff enhances the yield and solubility of BCR by optimizing the composition of the culture medium, induction conditions, and cultivation time. In addition, our labs employ various techniques such as transcription factor regulation and temperature adjustment to further increase protein expression levels.
4. BCR Protein Purification
Following expression, purifying the BCR protein is a critical step. Our scientists use multiple purification techniques, including affinity chromatography, ion-exchange chromatography, and gel filtration chromatography, in conjunction with our efficient purification processes to obtain high-purity BCR protein. Additionally, we have implemented advanced purification monitoring technologies, such as online mass spectrometry and HPLC, to ensure that every step meets quality standards.
5. Functional Validation
Finally, the purified BCR protein undergoes rigorous functional validation. Our team confirms the functionality and stability of the obtained BCR through techniques such as ELISA, flow cytometry, and bioactivity assays (cell binding experiments), ensuring its suitability for different research applications.
The processes of expressing and purifying BCRs can be complicated and time-consuming, hindering research progress. To address this issue, Creative Biolabs has established a specialized platform for BCR expression and purification services. If you are interested in our BCR expression and purification services or have any questions, please feel free to contact us at any time.
For research use only. Not intended for any clinical use.
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