Antibody-Cytokine Fusion Production Services

As a leading Contract Research Organization (CRO) company, Creative Biolabs specializes in providing high-quality, custom-made antibody-cytokine fusion proteins to meet your specific needs. Till now, we offer a wide range of services including antibody-cytokine fusion protein design, production, purification, and characterization.

Platform

Antibody-cytokine fusions are innovative biopharmaceuticals that combine the targeting specificity of antibodies with the potent biological activities of cytokines. Creative Biolabs offers production services for these fusion proteins. Our services typically include protein expression, purification, characterization, and assay development.

Fig.1 Antibody-cytokine Fusions Production Process. (Creative Biolabs Original)

Services

At Creative Biolabs, we are well-versed in the latest technologies and techniques, ensuring that we can deliver the best possible results for your research. By leveraging our advanced technology and in-depth knowledge, we can provide you with innovative antibody-cytokine fusion solutions that meet your special requirements. The key steps involved in the production of antibody-cytokine fusions include:

1. Designing antibody-fusion protein:

Our scientists will help clients select an appropriate antibody clone that targets a specific antigen of interest and a cytokine that has the desired biological activity.

2. Cloning and expression of antibody-fusion protein:

The gene encoding the fusion protein is then cloned into an expression vector and transfected into a suitable host cell line, such as HEK293 cells or CHO cells. The cells are then cultured in bioreactors to produce the fusion protein.

3. Purification of antibody-fusion protein:

The fusion protein is purified from the cell culture supernatant using various chromatography techniques, such as protein A or protein G affinity chromatography. The purity and identity of the fusion protein are confirmed using methods such as SDS-PAGE and Western blot analysis.

4. Characterization of the fusion protein:

The fusion protein is characterized to ensure that it has the desired biological activity and specificity. We will test the binding affinity of the antibody to its target antigen and the cytokine activity of the fusion protein.

5. In vitro and in vivo testing:

The fusion protein is tested in vitro to assess its effects on target cells, such as immune cells or cancer cells. In vivo studies may also be conducted to evaluate the efficacy of the fusion protein in animal models of disease.

6. Data analysis and reporting:

The results of the characterization and testing of our antibody-fusion protein are analyzed and compiled into a comprehensive report. Recommendations for further development or optimization of the fusion protein may also be provided.

Fig.2 The Biological Activity Analysis of a Single Chain Diabody-IL21 Fusion.^1,2

Features

Creative Biolabs has a well-established technical process for the design, production, validation, and quality control of our antibody-cytokine fusion protein/conjugate products.

Our expert team of scientists work closely with clients to design custom antibody-cytokine fusion proteins/conjugates that meet their specific needs and target antigens. We utilize the latest bioinformatics tools and molecular modeling techniques to design fusion proteins with optimal binding affinity and stability.

Once the design is finalized, our production facilities are capable of producing large quantities of antibody-cytokine fusion proteins/conjugates using advanced bioprocessing techniques. Before delivery, all antibody-cytokine fusion proteins/conjugates undergo rigorous validation testing to ensure their efficacy and safety. Our validation process includes biochemical and biophysical characterization to confirm the purity and stability of the product. Meanwhile, our QC team conducts thorough testing to verify the identity, purity, and potency of each product before it is released to the client.

Currently, our antibody-cytokine fusion proteins/conjugates have a wide range of applications in the fields of immunotherapy, oncology, and autoimmune diseases. Whether it is for targeted cancer therapy or immunomodulatory treatment, our fusion proteins are designed to deliver precise and effective results.

• Guarantee high success rate and fast delivery speed;
• Professional technical support, ensure strong, thoughtful, and timely pre-sales and after-sales service support;
• Complete upstream and downstream services, ranging from antibody preparation, antibody sequencing, antibody labeling, and modification, saving customers valuable time and funds;
• Reasonable price, according to the comprehensive analysis of customer needs, to provide customers with satisfactory results.

If you are interested in learning more about our antibody-cytokine fusion services or would like to place an order, please don't hesitate to reach out to us. Creative Biolabs is here to support your research goals and help you achieve success in your projects. Contact us immediately to get started!

References

1. Di Nitto, Cesare, et al. "Design and characterization of novel antibody-cytokine fusion proteins based on interleukin-21." Antibodies 11.1 (2022): 19.
2. under Open Access License CC BY 4.0, without modification.

For research use only. Not intended for any clinical use.