ADCC Enhanced Antibody Products

The Necessary to Improve Antibodies' ADCC

Antibody-dependent cellular cytotoxicity (ADCC) is an immune response wherein immune cells, like natural killer (NK) cells, are activated to destroy target cells that have antibodies attached to their surface. While ADCC is crucial for the effectiveness of cancer antibodies, many approved drugs exhibit lower-than-expected ADCC due to competition with nonspecific IgG for binding to FcγIIIa receptors on NK cells. Consequently, enhancing antibody ADCC is a key strategy in developing highly effective antibody-based therapies for cancer.

Fig.1 ADCC enhancement strategies.^1,3

Customized ADCC Enhanced Antibody Production Service at Creative Biolabs

Creative Biolabs offers comprehensive ADCC-enhanced antibody production services, leveraging advanced technologies to optimize antibody production. By precisely engineering the Fc region through amino acid sequence alteration or Fc-linked glycan modification, we significantly enhance antibody binding affinity to Fcγ receptors (FcγR), thereby triggering potent ADCC. In addition to engineering the antibodies themselves, we also offer services to engineer host cells by introducing small interfering RNA to customize ADCC-enhanced cell lines for antibody expression.

To ensure the excellent quality of the ADCC-enhanced antibodies, we employ a comprehensive set of strict quality control techniques. We provide specialized services to detect ADCC-enhanced antibodies, such as FcγR binding detection, ADCC assay, etc. In brief, we are dedicated to delivering high-quality, customized ADCC-enhanced antibodies to meet our customers' specific timelines and requirements.

Fig.2 Our service process.

ADCC-enhancement Strategies

Fc protein engineering and Fc glycan engineering provide powerful strategies for enhancing Fc-mediated ADCC. Here we provide several strategies to engineer the Fc domain of antibodies to improve ADCC:

• Modifying the Fc portion of the antibodies through site-directed mutagenesis, altering Fc domain glycosylation, or removing Fc domain fucosylation.
• Asymmetrically manipulating the Fc region of antibodies to form heterodimers of different heavy chains.

Selective ADCC Enhanced Cell Expression Systems

At Creative Biolabs, we offer a diverse range of ADCC-enhanced cell lines to support the development of these highly effective antibodies:

• ADCC enhanced CHOK1 cell line
• ADCC enhanced HEK293 cell line

Advantages of Our ADCC Enhanced Antibody Production Service

• Selective engineering strategies to improve ADCC.
• Comply with industry regulatory specifications.
• Validated ADCC enhanced cell line to choose from.
• High-quality, high-yield, and flexible production of ADCC-enhanced antibodies.
• Flexible and customizable service options.

Data Display

Summary: In this research, anti-CD19 antibodies were modified to improve ADCC, and assessed their potential to trigger ADCC. The findings revealed that the engineered antibodies displayed a stronger binding affinity to the FcγRIIIA and exhibited greater ADCC activity when compared to the original, unmodified anti-CD19 antibodies.

Fig.3 The assessment of engineered CD19 antibodies on FcγRIIIA binding and induction of ADCC.^2,3

As a premier provider of antibody solutions, Creative Biolabs is committed to advancing drug discovery and preclinical research. Our specialized expertise in ADCC-enhanced antibody production guarantees the supply of top-tier antibodies that fulfill the rigorous requirements of contemporary biomedical research. For further information about our ADCC-enhanced antibody products and production service, please reach out to us without delay.

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For Research Use Only. Not For Clinical Use.