APC-H-2Kd/Influenza A virus NP (TYQRTRALV) MHC Tetramer
CAT#: MHC-LC550
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of Influenza A-derived peptide of TYQRTRALV sequence covering 147-155 and H-2Kd molecule. The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Specifications
- Allele
- H-2Kd
- Class
- Class I
- MHC Species
- Mouse
- Antigen
- Influenza A virus NP
- Antigen Species
- Influenza A virus
- Peptide
- TYQRTRALV
- Range
- 147-155
- Conjugate
- APC
- Application
- FCM
Target
- Antigen Introduction
- Influenza A virus causes influenza in birds and some mammals, and is the only species of influenza virus A. Influenza virus A is a genus of the Orthomyxoviridae family of viruses. Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans. Occasionally, viruses are transmitted from wild aquatic birds to domestic poultry, and this may cause an outbreak or give rise to human influenza pandemics.
- Alternative Names
- Influenza A virus NP; Influenza A virus Nucleoprotein
- Gene ID
- 956531
- UniProt ID
- P03466
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Great For Influenza Research
This H-2Kd tetramer loaded with the Influenza A NP peptide TYQRTRALV worked perfectly. We were able to identify virus-specific CD8 T cells in our infected mouse models. The APC fluorescence was bright and the background staining was negligible.
Robust Viral Antigen Detection
We utilized this reagent to track the immune response to the viral nucleoprotein. The tetramer complex is stable and binds efficiently to the specific T cells, making it a reliable tool for our longitudinal studies on viral immunity.
Q&As
-
What viral protein is the peptide in this tetramer derived from?
A: The peptide TYQRTRALV (residues 147-155) is derived from the Nucleoprotein (NP) of Influenza A virus. This protein encapsulates the viral RNA and is a key target for cytotoxic T cell responses.
-
Which mouse MHC allele is this peptide restricted to?
A: This peptide is restricted to the H-2Kd allele. The tetramer is constructed with this mouse MHC Class I molecule to detect specific CD8 T cells in BALB/c or other H-2Kd positive mouse strains.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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