Recombinant Dromedary Anti-BACE1 Single Domain Antibody (B9) (CAT#: HPAB-0354-YC-VHH)

Described are single domain antibodies with a specificity for BACE1. More specifically, described are single variable-domain antibodies derived from camelids that bind to BACE1 and are capable of inhibiting the activity of BACE1. The antibodies can be used for research and medical applications. Specific applications include the use of BACE1-specific antibodies for the treatment of Alzheimer's disease.


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  • Published Data
  • Gene Expression
  • Datasheet
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Inhib

Figure 1 The inhibition effects of different sdAbs (10 μM) on BACE1 activity in FRET assay at a concentration of 10 μM.

Figure 1 The inhibition effects of different sdAbs (10 μM) on BACE1 activity in FRET assay at a concentration of 10 μM.

Activ

Figure 2 Dose-response curve of sdAbs B9 on BACE1 cleavage activity in FRET assay using a small peptide substrate.

Figure 2 Dose-response curve of sdAbs B9 on BACE1 cleavage activity in FRET assay using a small peptide substrate.

ELISA

Figure 3 Dose-response curve of Nb_B9 on BACE1 cleavage activity in MBP-ELISA using a big peptide substrate. In this cell-free enzymatic assay, Nb_B9 significantly inhibits BACE1 activity.

Figure 3 Dose-response curve of Nb_B9 on BACE1 cleavage activity in MBP-ELISA using a big peptide substrate. In this cell-free enzymatic assay, Nb_B9 significantly inhibits BACE1 activity.

WB

Figure 4 VHHs Nb_B9 and Nb_4A2 inhibit BACE1 cleavage of APPwt in primary cultured mouse neurons, as reflected in a decrease of Aβ, sAPPβ and CTFβ.

Figure 4 VHHs Nb_B9 and Nb_4A2 inhibit BACE1 cleavage of APPwt in primary cultured mouse neurons, as reflected in a decrease of Aβ, sAPPβ and CTFβ.

Primary cultured neurons from wild-type mice were transduced with APPwt by Semliki Forest Virus (SFV), and then treated with purified Nb_B9 and Nb_4A2 by adding to the medium at a final concentration of 20 μM (VHHs were first dissolved in PBS), neurons treated with PBS were used as a negative control. After a 16-hour treatment, conditioned medium and cell extract were analyzed by Western blot for APP-FL, CTFβ, CTFα, Aβ, sAPPβ and sAPPα/β.

Inhib

Figure 5 Dose response curve of VHHs Nb_B9 inhibiting BACE1 in primary cultured mouse neurons established by metabolic labeling assays after a 6-hour treatment.

Figure 5 Dose response curve of VHHs Nb_B9 inhibiting BACE1 in primary cultured mouse neurons established by metabolic labeling assays after a 6-hour treatment.

Primary cultured neurons from wild-type mice were transduced with APPwt by Semliki Forest Virus (SFV), and treated with purified VHH B9 by adding to the medium serial dilutions (VHH B9 was first dissolved and diluted in PBS). Neuron cultures were metabolic labeled for 6 hours, APP-FL and CTFβ from cell extracts were analyzed by phosphorimaging, while sAPPβ, Aβ and sAPPα from conditioned medium were analyzed by Western blot.


Specifications

  • Immunogen
  • Recombinant human BACE1
  • Host Species
  • Dromedary
  • Derivation
  • Phage display library
  • Type
  • Dromedary VHH
  • Specificity
  • Human BACE1
  • Species Reactivity
  • Human, Mouse
  • Clone
  • B9
  • Applications
  • ELISA, Western Blot, Inhibition, Activation
  • Related Disease
  • Alzheimer's disease

Applications

  • Application Notes
  • The antibody was validated for ELISA, Western Blot, Inhibition, Activation. For details, refer to Published Data.

Target

  • Alternative Names
  • Beta-Site APP-Cleaving Enzyme 1; Membrane-Associated Aspartic Protease 2; Beta-Site APP Cleaving Enzyme 1; Aspartyl Protease 2; EC 3.4.23.46; Memapsin-2; Asp 2; BACE; ASP2; Beta-Site Amyloid Beta A4 Precursor Protein-Cleaving Enzyme; Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1; Transmembrane Aspartic Proteinase Asp2; Beta-Secretase 1 Precursor Variant 1; Beta-Site APP-Cleaving Enzyme; APP Beta-Secretase; EC 3.4.23; KIAA1149; HSPC104

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "BACE1"

Single-domain Antibody

CAT Product Name Application Type
NAB-145-VHH Recombinant Anti-Human BACE1 VHH Single Domain Antibody IHC, IP, FC, Neut, FUNC Llama VHH
PNBL-032 Recombinant Anti-Human BACE1 VHH Single Domain Antibody (XA4813) WB, ELISA, FuncS Llama VHH

Recombinant Antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-036CQ Mouse Anti-BACE1 Recombinant Antibody (clone CBL278) ELISA, IP, Neut Mouse IgG1
NEUT-037CQ Mouse Anti-BACE1 Recombinant Antibody (clone CBL696) IP, Neut Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0315 Hi-Affi™ Rabbit Anti-BACE1 Recombinant Antibody (clone DS315AB) WB, IP Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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