Described are single domain antibodies with a specificity for BACE1. More specifically, described are single variable-domain antibodies derived from camelids that bind to BACE1 and are capable of inhibiting the activity of BACE1. The antibodies can be used for research and medical applications. Specific applications include the use of BACE1-specific antibodies for the treatment of Alzheimer's disease.
Figure 1 The inhibition effects of different sdAbs (10 μM) on BACE1 activity in FRET assay at a concentration of 10 μM.
Figure 2 Dose-response curve of sdAbs B9 on BACE1 cleavage activity in FRET assay using a small peptide substrate.
Figure 3 Dose-response curve of Nb_B9 on BACE1 cleavage activity in MBP-ELISA using a big peptide substrate. In this cell-free enzymatic assay, Nb_B9 significantly inhibits BACE1 activity.
Figure 4 VHHs Nb_B9 and Nb_4A2 inhibit BACE1 cleavage of APPwt in primary cultured mouse neurons, as reflected in a decrease of Aβ, sAPPβ and CTFβ.
Primary cultured neurons from wild-type mice were transduced with APPwt by Semliki Forest Virus (SFV), and then treated with purified Nb_B9 and Nb_4A2 by adding to the medium at a final concentration of 20 μM (VHHs were first dissolved in PBS), neurons treated with PBS were used as a negative control. After a 16-hour treatment, conditioned medium and cell extract were analyzed by Western blot for APP-FL, CTFβ, CTFα, Aβ, sAPPβ and sAPPα/β.
Figure 5 Dose response curve of VHHs Nb_B9 inhibiting BACE1 in primary cultured mouse neurons established by metabolic labeling assays after a 6-hour treatment.
Primary cultured neurons from wild-type mice were transduced with APPwt by Semliki Forest Virus (SFV), and treated with purified VHH B9 by adding to the medium serial dilutions (VHH B9 was first dissolved and diluted in PBS). Neuron cultures were metabolic labeled for 6 hours, APP-FL and CTFβ from cell extracts were analyzed by phosphorimaging, while sAPPβ, Aβ and sAPPα from conditioned medium were analyzed by Western blot.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
NAB-145-VHH | Recombinant Anti-Human BACE1 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
PNBL-032 | Recombinant Anti-Human BACE1 VHH Single Domain Antibody (XA4813) | WB, ELISA, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2939z | Mouse Anti-BACE1 Recombinant Antibody (clone 18H11) | WB, ELISA, FC, ICC | Mouse IgG1 |
PABL-408 | Human Anti-BACE1 Recombinant Antibody (PABL-408) | ELISA, Block, Neut | Human IgG |
HPAB-0285-YC | Mouse Anti-BACE1 Recombinant Antibody (HPAB-0285-YC) | ELISA, FuncS | Mouse IgG |
VS3-QX99 | Mouse Anti-BACE1 Recombinant Antibody (clone 7H4) | WB, IP, IF, IHC | Mouse IgG1 |
VS3-XY114 | Mouse Anti-BACE1 Recombinant Antibody (clone 3C1C3) | ELISA, WB, ICC, FC | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-405 | Human Anti-BACE1 Recombinant Antibody; Fab Fragment (PFBL-405) | ELISA, Block, Neut | Human Fab |
HPAB-0285-YC-F(E) | Mouse Anti-BACE1 Recombinant Antibody; Fab Fragment (HPAB-0285-YC-F(E)) | ELISA, FuncS | Mouse Fab |
HPAB-0672-CN-F(E) | Mouse Anti-BACE1 Recombinant Antibody (clone M2-1003); Fab Fragment | ELISA, FC, IHC, Neut | Mouse Fab |
HPAB-0673-CN-F(E) | Mouse Anti-BACE1 Recombinant Antibody (clone M2-1000); Fab Fragment | ELISA, FC, IHC, Neut | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-405 | Human Anti-BACE1 Recombinant Antibody; scFv Fragment (PSBL-405) | ELISA, Block | Human scFv |
HPAB-0285-YC-S(P) | Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (HPAB-0285-YC-S(P)) | ELISA, FuncS | Mouse scFv |
HPAB-0672-CN-S(P) | Mouse Anti-BACE1 Recombinant Antibody (clone M2-1003); scFv Fragment | ELISA, FC, IHC, Neut | Mouse scFv |
HPAB-0673-CN-S(P) | Mouse Anti-BACE1 Recombinant Antibody (clone M2-1000); scFv Fragment | ELISA, FC, IHC, Neut | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1156CL | Mouse Anti-BACE1 Recombinant Antibody (TAB-1156CL) | WB, IP, Inhib | Mouse IgG |
TAB-1153CL-F(E) | Mouse Anti-BACE1 Recombinant Antibody; Fab Fragment (TAB-1153CL-F(E)) | ELISA | Mouse Fab |
TAB-1154CL-F(E) | Mouse Anti-BACE1 Recombinant Antibody; Fab Fragment (TAB-1154CL-F(E)) | WB, ELISA | Mouse Fab |
TAB-1155CL-F(E) | Mouse Anti-BACE1 Recombinant Antibody; Fab Fragment (TAB-1155CL-F(E)) | ELISA, Inhib, FuncS | Mouse Fab |
TAB-1156CL-F(E) | Mouse Anti-BACE1 Recombinant Antibody; Fab Fragment (TAB-1156CL-F(E)) | ELISA, Inhib, FuncS | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-036CQ | Mouse Anti-BACE1 Recombinant Antibody (clone CBL278) | ELISA, IP, Neut | Mouse IgG1 |
NEUT-037CQ | Mouse Anti-BACE1 Recombinant Antibody (clone CBL696) | IP, Neut | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0315 | Hi-Affi™ Rabbit Anti-BACE1 Recombinant Antibody (clone DS315AB) | WB, IP | Rabbit IgG |
There are currently no Customer reviews or questions for HPAB-0354-YC-VHH. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.