Recombinant single domain antibody specifically binds to C. jejuni flagella, expressed in Chinese Hamster Ovary cells(CHO).
Figure 1 SPR analysis of the binding of the monomeric and pentameric VHHs to antigen.
Sensorgram overlays represent the binding of 28, 42, 56, 70, 140 and 190 nM of FlagV6M to 700 RUs of biotinylated flagellin captured on an SA sensorchip.
Figure 2 Epitope mapping of anti-flagella antibodies by simultaneous binding of flagella-specific VHHs to the target antigen in SPR co-injection experiments.
For the antigen, 60–100 µl of each VHH, at a concentration 50× its KD value, were injected over 600–700 RUs of immobilized flagella at 30 µl/min. FlagV1 and FlagV6 appeared to bind distinct, non-overlapping epitopes since the signal approximately doubled with the second injection.
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For research use only. Not intended for any clinical use.