Anti-C. jejuni Therapeutic Antibody (FlagV1M) (CAT#: MRO-118CT)

Recombinant single domain antibody specifically binds to C. jejuni flagella, expressed in Chinese Hamster Ovary cells(CHO).

  • Published Data
  • Datasheet
  • MSDS
  • COA
ELISA

Figure 1 ELISA analysis of binding of the monomeric and pentameric VHHs to C. jejuni surface antigens.

Various concentrations (ranging from 10 to 0.0001 µg/ml) of the monomeric (FlagV1M) and pentameric (FlagV1P) forms of a flagella-specific VHH were used in ELISA.

Riazi, A., Strong, P. C., Coleman, R., Chen, W., Hirama, T., van Faassen, H., ... & Ghahroudi, M. A. (2013). Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens. PloS one, 8(12), e83928.

SPR

Figure 2 SPR analysis of the binding of the monomeric and pentameric VHHs to antigen.

Sensorgram overlays represent the binding of 28, 42, 56, 70, 140 and 190 nM of FlagV1M to 700 RUs of biotinylated flagellin captured on an SA sensorchip.

Riazi, A., Strong, P. C., Coleman, R., Chen, W., Hirama, T., van Faassen, H., ... & Ghahroudi, M. A. (2013). Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens. PloS one, 8(12), e83928.

SPR

Figure 3 SPR analysis of the binding of the monomeric and pentameric VHHs to antigen.

The sensograms of FlagV1M (in red) and FlagV1P (in blue) were overlaid to show that a slower off-rate is obtained as a result of pentamerization indicating an increase in the functional affinity (avidity) of FlagV1P.

Riazi, A., Strong, P. C., Coleman, R., Chen, W., Hirama, T., van Faassen, H., ... & Ghahroudi, M. A. (2013). Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens. PloS one, 8(12), e83928.

SPR

Figure 4 Epitope mapping of anti-flagella antibodies by simultaneous binding of flagella-specific VHHs to the target antigen in SPR co-injection experiments.

For the antigen, 60–100 µl of each VHH, at a concentration 50× its KD value, were injected over 600–700 RUs of immobilized flagella at 30 µl/min. FlagV1 and FlagV6 appeared to bind distinct, non-overlapping epitopes since the signal approximately doubled with the second injection.

Riazi, A., Strong, P. C., Coleman, R., Chen, W., Hirama, T., van Faassen, H., ... & Ghahroudi, M. A. (2013). Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens. PloS one, 8(12), e83928.


Specifications

  • Immunogen
  • C. jejuni flagella
  • Host Species
  • Llama
  • Type
  • Single domain antibody
  • Specificity
  • C. jejuni flagella
  • Species Reactivity
  • C. jejuni
  • Clone
  • FlagV1M
  • Applications
  • ELISA, Surface plasmon resonance
  • Related Disease
  • Enteric and extraintestinal illnesses

Applications

  • Application Notes
  • The antibody was validated for ELISA, Surface plasmon resonance. For details, refer to Published Data.

Target

  • Alternative Names
  • Campylobacter jejuni; C. jejuni

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "C. jejuni"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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