Rabbit Anti-Hypusine Recombinant Antibody (clone Hpu24) (CAT#: PABL-202)
Recombinant Rabbit Antibody (Hpu24) is capable of binding to Hypusine, expressed in Chinese Hamster Ovary cells (CHO).
Specific Inquiry
Figure 1 Anti-hypusine antibodies bound to hypusinated peptides independent of flanking sequences as shown by ELISA
Figure 2 Anti-hypusine antibodies specifically bound to hypusine in the native intact protein eIF5A as shown by Western blot analysis.
Figure 3 Representative biosensor sensorgrams for FabHpu24 or FabHpu24.B binding to immobilized Pl-Hpu peptide or Pl-deoxyHpu peptide.
Specifications
- Immunogen
- Human Hypusine
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Hypusine
- Species Reactivity
- Human
- Clone
- Hpu24
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for ELISA and WB. For details, refer to published data.
Target
- Alternative Names
- Hypusine
Related Resources
Tan, Thomas CJ, et al. "Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8+ T lymphocytes." Nature Communications 13.1 (2022): 7796.
This study investigates the essential role of the translation factor eIF5a in the production of interferon-gamma (IFNγ) and the regulation of the cell cycle in primary CD8+ T lymphocytes. The research demonstrates that eIF5a is dynamically regulated through post-translational modification in naïve CD8+ T cells upon activation, facilitating the translation of specific subsets of proteins crucial for long-term survival and effector functions. The study underscores the importance of eIF5a in the coordination of immune responses by controlling the translation of mRNAs that regulate proliferation and key effector functions, including the production of IFNγ.
At Creative Biolabs, we supplied the hypusine antibody Hpu24 (Cat#: PABL-202) used in flow cytometry and Western blotting analyses. Our products were crucial in enabling the detailed examination of eIF5a's role in T cell activation and function. This study's significance lies in its potential to uncover new therapeutic targets for enhancing immune responses and treating conditions related to immune dysfunction, highlighting our commitment to advancing immunological research and innovative solutions.
More Infomation
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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scFv Fragment Antibody
Fab Fragment Antibody
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-202 | Rabbit Anti-Hypusine Recombinant Antibody (clone Hpu24); scFv Fragment | WB, ELISA, FuncS | Rabbit scFv |
| PSBL-577 | Rabbit Anti-Hypusine Recombinant Antibody (clone Hpu98); scFv Fragment | WB, ELISA, FuncS | Rabbit scFv |
| HPAB-2335LY-S(P) | Rabbit Anti-Hypusine Recombinant Antibody; scFv Fragment (HPAB-2335LY-S(P)) | ELISA, FuncS | Rabbit scfv |
| HPAB-2336LY-S(P) | Rabbit Anti-Hypusine Recombinant Antibody; scFv Fragment (HPAB-2336LY-S(P)) | ELISA, FuncS | Rabbit scfv |
| HPAB-2337LY-S(P) | Rabbit Anti-Hypusine Recombinant Antibody; scFv Fragment (HPAB-2337LY-S(P)) | ELISA, FuncS | Rabbit scfv |
Customer Reviews and Q&As
Customer Review
Q&As
-
Ideal for Flow Cytometry
The Anti-Hypusine Recombinant Antibody (clone Hpu24) has proven to be incredibly reliable in our flow cytometry and immunofluorescence applications. The results are always high-quality and reproducible, with strong affinity and specificity for Hypusine. It has become a staple in our lab for various experiments, providing us with the confidence to proceed with complex assays. The robust performance of this antibody has significantly enhanced the quality of our data. -
Great for Western Blots
We incorporated this antibody into our Western blot assays, and it has been fantastic. The specificity and sensitivity for Hypusine are impressive, consistently delivering clear and precise results. It has made our research process smoother and more reliable. -
Reliable in Multiple Applications
This antibody has been reliable across various applications, including flow cytometry and immunofluorescence. The high-quality, reproducible results and strong affinity for Hypusine have significantly benefited our experiments, making it an indispensable tool in our lab. The consistent performance has greatly enhanced our data quality.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-202, RRID: AB_2877637)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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