Recombinant Anti-Mouse Abcb1a VHH Single Domain Antibody (Nb592) (CAT#: PNBL-079)

Anti-Mouse Abcb1a VHH Single Domain Antibody is a recombinant protein produced in E. coli.

  • Published Data
  • Datasheet
  • MSDS
  • COA
Inhib

Figure 1 Nb592 is a strong inhibitor of P-gp’s ATPase activity.

Nb592 inhibits verapamil-stimulated ATPase activity with an IC50 of 520 ± 57 nM. Data points indicate the average activity ± SEM from four independent experiments, relative to P-gp's activity in the absence of Nb592.

Ward, A. B., Szewczyk, P., Grimard, V., Lee, C. W., Martinez, L., Doshi, R., ... & Swartz, D. J. (2013). Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Proceedings of the National Academy of Sciences, 110(33), 13386-13391.

Inhib

Figure 2 Nb592 is a strong inhibitor of P-gp’s ATPase activity.

Inhibition of verapamil-stimulated ATPase activity in the presence of 5 mM DTT, without or with 7.5 μM Nb592 (n = 4).

Ward, A. B., Szewczyk, P., Grimard, V., Lee, C. W., Martinez, L., Doshi, R., ... & Swartz, D. J. (2013). Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Proceedings of the National Academy of Sciences, 110(33), 13386-13391.

Inhib

Figure 3 Nb592 is a strong inhibitor of P-gp’s ATPase activity.

Nb592 prevents vanadate-induced 8-azido-[α³²P]-ADP trapping in P-gp's catalytic sites. P-gp was incubated with 100 μM verapamil (VER) and 200 μM orthovanadate (Vi) in the 8-azido-[α³²P]-ATP hydrolysis/trapping reaction as indicated above the lanes.

Ward, A. B., Szewczyk, P., Grimard, V., Lee, C. W., Martinez, L., Doshi, R., ... & Swartz, D. J. (2013). Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Proceedings of the National Academy of Sciences, 110(33), 13386-13391.


Specifications

  • Host Species
  • Llama
  • Derivation
  • Llama
  • Type
  • Llama VHH
  • Species Reactivity
  • Mouse
  • Clone
  • Nb592
  • Applications
  • Inhib, WB, ELISA, FuncS

Applications

  • Application Notes
  • The antibody was validated for Inhibition, Western Blot, ELISA, Functional Assay. For details, refer to Published Data.

Target

  • Alternative Names
  • Pgp; Mdr3; P-gp; Pgy3; Abcb4; Evi32; Mdr1a; Pgy-3; mdr-3

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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