Anti-P. aeruginosa exopolysaccharide Psl Recombinant Antibody (Cam-004) (MRO-161MZ) (CAT#: MRO-161MZ)

Figure 1 Functional activity screening of antibodies derived from phage scFv patient libraries.

In vitro functional screens included OPK assays and cell attachment assays using the lung epithelial cell line A549. R347, an isotype-matched human mAb that does not bind P. aeruginosa antigens, was used as a negative control.

Identification of broadly protective human antibodies to Pseudomonas aeruginosa exopolysaccharide Psl by phenotypic screening

Figure 2 Psl mAb Cam-003 protects mice against lethal challenge in a P. aeruginosa acute pneumonia model.

Animals were monitored for survival between 72 and 120 h after infection. In all experiments, PBS and R347 served as negative controls. A PBS control was not tested in this experiment because previous results indicated no difference in survival versus mice treated with R347. In its place, we used challenge with PAO1ΔpslA as an additional control. Results are represented as Kaplan-Meier survival curves; differences in survival were calculated by the log-rank test for Cam-003 versus R347.

Identification of broadly protective human antibodies to Pseudomonas aeruginosa exopolysaccharide Psl by phenotypic screening