H-2Ld/Human P815A P1A (LPYLGWLVF) MHC Tetramer
CAT#: MHC-LC204
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of P815A P1A-derived peptide of LPYLGWLVF sequence covering 35-43 and H-2Ld molecule. The pMHC tetramer recognizes mouse CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Specifications
- Allele
- H-2Ld
- Class
- Class I
- MHC Species
- Mouse
- Antigen
- P815A P1A
- Antigen Species
- Human
- Peptide
- LPYLGWLVF
- Range
- 35-43
- Conjugate
- PE
- Application
- FCM
Target
- Antigen Introduction
- Tumor rejection antigen P1A is one of the first tumor rejection antigens identified in the mouse. Its expression is tightly regulated and is restricted to immune privileged organs such as testis and some lineages of cancer cells including myeloma cells.
- Alternative Names
- Trap1a; tumor rejection antigen P1A; Tra-P1A
- Gene ID
- 22037
- UniProt ID
- P19473
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Excellent for P815A Research
This PE-labeled tetramer was perfect for detecting mouse CD8 T cells specific to the P815A P1A peptide LPYLGWLVF. It allowed us to accurately track the immune response to this tumor rejection antigen.
Stable H-2Ld Reagent
The product quality was high, with stable complexes that provided consistent staining in flow cytometry. We successfully analyzed T cells targeting the 35-43 peptide region of Trap1a in our experimental models.
Q&As
-
What is the target antigen for this H-2Ld tetramer?
A: The target antigen is P815A P1A (Trap1a), a tumor rejection antigen. The tetramer is loaded with the peptide sequence LPYLGWLVF (amino acids 35-43) and is presented by H-2Ld.
-
What type of cells can be identified with this product?
A: This product is designed to identify mouse CD8 T cells. The H-2Ld MHC Class I molecule presents the peptide to T cells, allowing for their detection by flow cytometry using the PE label.
View the frequently asked questions answered by Creative Biolabs Support.
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