L27 domain scaffold protein anti-Human CASK
CAT#: SLT-L119
The L27 domain that bound CASK was isolated from the library using Yeast Two-hybrid techonology. After affinity maturation, L27 domain variants were identified having affinities for CASK as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23857/if_selected.jpg
Normal Tissue
Figure 2 Small intestine
High expression in small intestine
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23857/52781_A_6_2_val_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1949/5081_A_9_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1949/5081_A_5_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1949/5081_A_7_8.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1949/5081_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1949/5081_A_8_4.jpg
Normal Tissue
Figure 8 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1949/5081_A_3_3.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147044-CASK
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Specifications
- Scaffold Name
- L27 domain
- Origin
- PSD-95 protein
- Target
- CASK
- Species Reactivity
- Human
- Expression Host
- E. coli
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
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Effective Synapse Marker
We are studying proteins located at synapses in the brain, and this L27 domain is a great marker for CASK. It binds well in our immunohistochemistry sections, helping us visualize the distribution of this calcium/calmodulin-dependent serine protein kinase.
Isolated Via Yeast Two-Hybrid
The Yeast Two-hybrid isolation method ensures that this protein interacts biologically with CASK. We have used it to pull down membrane-associated guanylate kinase complexes, aiding our research into X-linked mental retardation pathways.
Q&As
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What is the function of the target protein CASK?
A: CASK is a calcium/calmodulin-dependent serine protein kinase and a member of the MAGUK (membrane-associated guanylate kinase) protein family. These proteins function as scaffold proteins located at synapses in the brain.
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How was this L27 domain variant identified?
A: The L27 domain that binds CASK was isolated from the library using Yeast Two-hybrid technology. After affinity maturation, variants were identified with specific affinities for CASK as determined by biosensor technology.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "CASK"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0470 | Hi-Affi™ Rabbit Anti-CASK Recombinant Antibody (clone DS470AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1717-CN | Rabbit Anti-CASK Polyclonal Antibody (MRO-1717-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0325-XY319 | Anti-CASK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY992 | Anti-Human CASK Immunohistochemistry Kit | IHC |
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