What is the payload and how does it work?

The payload is DM1 (N2'-Deacetyl-N2'-(3-mercapto-1-oxopropyl)maytansine), a maytansinoid cytotoxin. It works by binding to tubulin at or near the vinblastine-binding site. This interferes with microtubule formation and depolymerizes existing microtubules, inducing mitotic arrest in the target cells.

How does the SMCC linker release the drug?

The SMCC linker is a noncleavable linker. The release of the cytotoxic drug does not occur through linker cleavage in the plasma. Instead, it is postulated to occur after the ADC is internalized via endocytosis and delivered to the lysosome, where the antibody is degraded proteolytically.

Anti-FcRH5 (clone 7D11)-SMCC-DM1 ADC

CAT#: ADC-161CL

This ADC product is composed of an anti-FcRH5 antibody (clone 7D11) conjugated via SMCC linker to DM1(Mu-anti-FcRH5 (7D11)-mcc-dm1). It has demonstrated a response in treatment by a MOA (Mechanism of Action) of microtubules depolymerizing.

Gene Expression

RNA Expression Figure 1 RNA cell line category: Group enriched (U-266/70, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143297-FCRL5

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Specifications

Antibody Overview
Mu-Anti-FcRH5 (7D11)

Clone
7D11

Linker
SMCC (N-succinimidyl 4-(Nmaleimidomethyl)cyclohexane-1-carboxylate)

Linker Class/Description
Class: Noncleavable linkers
Description: Noncleavable linker is considered noncleavable-meaning linker cleavage, and payload release does not depend on the differential properties between the plasma and some cytoplasmic compartments. Instead, the release of the cytotoxic drug is postulated to occur after internalization of the ADC via antigen-mediated endocytosis and delivery to lysosomal compartment, where the antibody is degraded to the level of amino acids through intracellular proteolytic degradation.

Drug
DM1 (N2'-Deacetyl-N2'-(3-mercapto-1-oxopropyl)maytansine)

Drug Class/Description
Class: Maytansinoid
Description: Maytansinoids are a group of cytotoxins structurally similar to rifamycin, geldanamycin, and ansatrienin. The eponymous natural cytotoxic agent maytansine is a 19-member lactam (ansa
macrolide) structure originally isolated from the Ethiopian shrub Maytenus ovatus. Maytansinoids can bind to tubulin at or near the vinblastine-binding site, which interfere the formation of microtubules and depolymerize already formed microtubules, inducing mitotic arrest in the intoxicated cells.

Target

Introduction
This gene encodes a member of the immunoglobulin receptor superfamily and the Fc-receptor like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-pass type I membrane protein and contains 8 immunoglobulin-like C2-type domains. This gene is implicated in B cell development and lymphomagenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]

Alternative Names
FCRL5; Fc receptor-like 5; CD307; FCRH5; IRTA2; BXMAS1; CD307e; PRO820; Fc receptor-like protein 5; fcR-like protein 5; fc receptor homolog 5; immune receptor translocation-associated protein 2; immunoglobulin superfamily receptor translocation associated 2 (IRTA2);

Gene ID
83416

UniProt ID
Q96RD9

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Breast cancer biomarkers at key points during disease progression

Excellent Murine Antibody Tool

This product, featuring the murine anti-FcRH5 clone 7D11, has been instrumental in our comparative studies. The conjugation to DM1 via the SMCC linker provided a stable molecule for our experiments. We appreciate the consistent quality of this ADC for investigating Fc receptor-like family members.

Stable Noncleavable Linker

Our research required a noncleavable linker to ensure payload release only occurs after lysosomal degradation. The SMCC linker in this ADC met our requirements perfectly, allowing us to accurately track the delivery of the microtubule-depolymerizing agent DM1 to the lysosomal compartment of the target cells.

Q&As

What is the payload and how does it work?

A: The payload is DM1 (N2'-Deacetyl-N2'-(3-mercapto-1-oxopropyl)maytansine), a maytansinoid cytotoxin. It works by binding to tubulin at or near the vinblastine-binding site. This interferes with microtubule formation and depolymerizes existing microtubules, inducing mitotic arrest in the target cells.
How does the SMCC linker release the drug?

A: The SMCC linker is a noncleavable linker. The release of the cytotoxic drug does not occur through linker cleavage in the plasma. Instead, it is postulated to occur after the ADC is internalized via endocytosis and delivered to the lysosome, where the antibody is degraded proteolytically.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Datasheet

Datasheet - ADC-161CL.pdf

MSDS

MSDS - ADC-161CL.pdf

COA

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