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    PE-B8/Human RPL18 (VPKLKVCAL) MHC Tetramer (CAT#: MHC-LC3424)

    This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of human RPL18 of peptide VPKLKVCAL covering 95-103 and B8 molecule. The pMHC tetramer recognizes CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.

    Specific Inquiry
    • Size:
    Custom Production

    MHC tetramer custom production provides a vital tool for researchers seeking to understand and manipulate T-cell immunity with exceptional precision. Please specify your requirements, including peptide sequence, MHC allele, and desired fluorophore etc. We will respond as soon as possible with a tailored solution.

    Peptide Sequence *:
    Allele Requested *:
    Antigen Species:
    Antigen Molecule:
    Sequence Position:
    Form Requested *:
    Conjugation *:
    Size *:
    Comments:

    Disclaimer: Please note that the MHC reagents we offered have been produced with strict quality control (including gene sequencing, affinity purification, SDS-PAGE analysis, and verification of biotinylation), but many have not been confirmed to stain or activate specific T cells.

    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line HEK 293
    Figure 1 IF staining of human cell line HEK 293
    (Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli, cytosol & endoplasmic reticulum.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46572/if_selected.jpg
    Figure 2 IHC staining of human pancreas
    Figure 2 IHC staining of human pancreas
    (Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46572/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Neuronal cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46572/116130_B_7_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46572/116130_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46572/116130_A_7_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in tubules
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46572/116130_A_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Round or early spermatids
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46572/116130_A_6_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Not detected
    Intensity: Weak
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46572/116130_A_9_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enriched (NB-4)
    Figure 9 RNA cell line category: Cell line enriched (NB-4)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000063177-RPL18/subcellular

    Specifications

    • Allele
    • B8
    • Class
    • Class I
    • MHC Species
    • Human
    • Antigen
    • RPL18
    • Antigen Species
    • Human
    • Peptide
    • VPKLKVCAL
    • Range
    • 95-103
    • Conjugate
    • PE
    • Application
    • FCM

    Target

    • Antigen Introduction
    • Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
    • Alternative Names
    • RPL18; 60S ribosomal protein L18; L18

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    IgG Antibody Products
    CAT Product Name Application Type
    MOB-0801MZ Recombinant Mouse Anti-Human RPL18 Antibody WB Mouse antibody

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