Z-domain of protein A scaffold protein anti-Mouse IgG1 Fab
CAT#: SZA-L234
This IgG1 Fab specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=10 nM) for IgG1 Fab as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specifications
- Scaffold Name
- Z-domain of protein A
- Origin
- Staphylococcal protein A
- Core Structure
- 3-α helixes
- Variable Regions
- 13 Residues on first and second helix surface
- Target
- IgG1 Fab
- Species Reactivity
- Mouse
- Expression Host
- E. coli
- Affinity Constant
- 10 nM
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
- Alternative Names
- Staphylococcal protein A; Z-domain of protein A; IgG1 Fab; IgG1; Fab
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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