Anti-DDIT3 (clone W24V176) Recombinant Antibody Coupled Liposome (VS-1024-FY177)
CAT#: VS-1024-FY177
The anti-DDIT3 antibody conjugated liposome plays an important role in melanoma, as DDIT3 is crucial in cellular stress response and tumor cell survival. By using this targeted liposome, DDIT3's function can be effectively disrupted, promoting apoptosis of tumor cells. Research findings suggest that this method may aid in developing new targeted therapeutic strategies. Future experiments will further validate its potential clinical applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58416/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68416/151563_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, hTERT-HME1, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175197-DDIT3
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More Infomation
Specifications
- Potential Clinical Applications
- Melanoma
Product Composition
- Clone
- W24V176
- Antibody Type
- IgG
- Antibody Host
- Mouse
- Antibody Reactivity
- Human
- Antibody Description
- This is a recombinant mouse monoclonal antibody, it was specifically designed to target and bind to DDIT3, a protein known to be involved in cellular stress responses. DDIT3, also referred to as C/EBP homologous protein (CHOP), plays a crucial role in the regulation of gene expression in response to endoplasmic reticulum stress and has implications in various physiological and pathological processes, including apoptosis, inflammation, and the development of certain diseases.
Product Property
- Storage
- See in the COA
- Storage Shelf Time
- See in the COA
Target Information
- Target
- DDIT3
- Introduction
- This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
- Alternative Names
- DNA Damage Inducible Transcript 3; C/EBP Zeta; Growth Arrest And DNA Damage-Inducible Protein GADD153; CCAAT/Enhancer-Binding Protein Homologous Protein; C/EBP-Homologous Protein 10; CHOP-10; GADD153; CHOP10
- Full Name
- DNA Damage Inducible Transcript 3
- Gene ID
- 1649
- UniProt ID
- P35638
- Sequence Similarities
- Belongs to the bZIP family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitinated, leading to its degradation by the proteasome. ( P35638-DDIT3_HUMAN )
Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity. ( P35638-DDIT3_HUMAN )
Modification sites at PhosphoSitePlus ( P35638 )
Modification sites at neXtProt ( NX_P35638 )
Glycosylation from GlyGen (P35638) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001181982.1; NP_001181983.1; NP_001181984.1; NP_001181985.1; NP_001181986.1
- Function
- Multifunctional transcription factor in endoplasmic reticulum (ER) stress response (PubMed:15322075, 15775988, 19672300).
Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress (PubMed:15322075, 15775988).
Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes (By similarity).
Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes (By similarity).
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MSDS
COA
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See other products for "DDIT3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2287CT | Recombinant Mouse anti-Human DDIT3 Monoclonal antibody (FQS4680) | WB, IP, ICC, IF, IHC-Fr, IHC-P | |
| VS3-XY570 | Mouse Anti-DDIT3 Recombinant Antibody (clone 4E8E9) | IHC, ICC, FC | Mouse IgG2a |
| VS3-FY381 | Recombinant Mouse Anti-DDIT3 Antibody (clone 1E1) | IHC | Mouse IgG |
| VS3-FY382 | Recombinant Mouse Anti-DDIT3 Antibody (clone 2B1) | WB, IHC | Mouse IgG |
| VS3-FY447 | Recombinant Rabbit Anti-DDIT3 Antibody (clone R02-6A3) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0951 | Hi-Affi™ Rabbit Anti-DDIT3 Recombinant Antibody (clone DS951AB) | IHC-P, WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY637 | Anti-DDIT3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1943 | Anti-Mouse DDIT3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1942 | Anti-Human DDIT3 Immunohistochemistry Kit | IHC |
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