Anti-DDX4 Antibody Prodrug, Protease Activated (2M1/2K4)
CAT#: VS-1025-YC141
The Anti-DDX4 Antibody Prodrug, Protease Activated (2M1/2K4) is an antibody prodrug comprised of a recombinant mouse antibody targeting human DDX4, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to DDX4 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to DDX4 effectively. DDX4 (VASA) is an RNA helicase whose oncological relevance is primarily as a marker for germ cell tumors. Its presence is used diagnostically to confirm the lineage of certain malignant neoplasms that originate from germ cell populations.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37763/1954_H3_62_selected.jpg
Normal Tissue
Figure 1 High expression in testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37764/110651_A_5_6.jpg
Normal Tissue
Figure 2 Pancreas
Exocrine glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37764/110651_A_1_3.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (BEWO, PC-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000152670-DDX4
❮
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Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human DDX4
- Target
- DDX4
- Species Reactivity
- Human
- Isotype
- IgG2a, kappa
- Clone
- 2M1/2K4
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 4; VASA; EC 3.6.4.13; EC 3.6.1
- Gene ID
- 54514
- UniProt ID
- Q9NQI0
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 2M1/2K4"
- CAT
- Product Name
See other products for "DDX4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-246CQ | Anti-Human DDX4 Recombinant Antibody (1E9) | ELISA, FC | Human antibody |
| TAB-247CQ | Anti-Human DDX4 Recombinant Antibody (1A12) | ELISA | Human antibody |
| TAB-246CQ-S(P) | Anti-Human DDX4 Recombinant Antibody scFv Fragment (1E9) | ELISA, FC | Human antibody |
| TAB-247CQ-S(P) | Anti-Human DDX4 Recombinant Antibody scFv Fragment (1A12) | ELISA, FC | Human antibody |
| TAB-246CQ-F(E) | Anti-Human DDX4 Recombinant Antibody Fab Fragment (1E9) | ELISA, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1366MZ | Recombinant Mouse Anti-Human DDX4 Antibody (clone NN0609-22H26) | WB | Mouse antibody |
| HPAB-0035CQ | Mouse Anti-DDX4 Recombinant Antibody (clone 2M1/2K4) | ELISA | Mouse IgG2a, κ |
| VS3-QX323 | Mouse Anti-DDX4 Recombinant Antibody (clone 2F9H5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-HM812 | Mouse Anti-DDX4 Recombinant Antibody (clone CBL194HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
| VS13-YC295 | Rabbit Anti-DDX4 Recombinant Antibody (VS13-YC295) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0960 | Hi-Affi™ Rabbit Anti-DDX4 Recombinant Antibody (clone DS960AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0035CQ-S(P) | Mouse Anti-DDX4 Recombinant Antibody (clone 2M1/2K4); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0035CQ-F(E) | Mouse Anti-DDX4 Recombinant Antibody (clone 2M1/2K4); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1960 | Anti-DDX4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1961 | Anti-Mouse DDX4 Immunohistochemistry Kit | IHC |
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