Anti-DEK Antibody Prodrug, Protease Activated (VS-1025-YC197)
CAT#: VS-1025-YC197
The Anti-DEK Antibody Prodrug, Protease Activated (VS-1025-YC197) is an antibody prodrug comprised of a recombinant mouse antibody targeting human DEK, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to DEK protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to DEK effectively. DEK is a chromatin-associated protein that functions as an oncogene when its gene is fused with CAN (DEK-CAN) in acute myeloid leukemia (AML). As a single protein, its overexpression is found in many solid tumors, promoting proliferation and resistance to apoptosis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57799/1171_B5_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15226/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54505/ihc_selected.jpg
Subcellular Location
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57799/ihc_selected.jpg
Subcellular Location
Figure 5 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15226/ihc_selected.jpg
Subcellular Location
Figure 6 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15226/620_H3_1_red_green.jpg
Subcellular Location
Figure 7 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15226/627_H3_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57799/149897_B_9_5.jpg
Normal Tissue
Figure 9 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57799/149897_A_9_3.jpg
Normal Tissue
Figure 10 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57799/149897_A_8_4.jpg
Normal Tissue
Figure 11 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57799/149897_A_7_5.jpg
Normal Tissue
Figure 12 Testis
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57799/149897_A_4_6.jpg
Normal Tissue
Figure 13 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57799/149897_A_8_8.jpg
RNA Expression
Figure 14 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000124795-DEK
❮
❯
Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human DEK
- Target
- DEK
- Species Reactivity
- Human
- Isotype
- IgG1
- Epitope
- AA 16-50, CQPASEKEPEMPGPREESEEEEDEDDEEEEEEEKGK
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein G purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases. Two transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- DEK Proto-Oncogene; DEK Oncogene; D6S231E
- Gene ID
- 7913
- UniProt ID
- P35659
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Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1283z | Mouse Anti-DEK Recombinant Antibody (clone 28G9) | WB, ELISA, IF, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0970 | Hi-Affi™ Rabbit Anti-DEK Recombinant Antibody (clone DS970AB) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0165-CN | Mouse Anti-DEK Recombinant Antibody (HPAB-0165-CN) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0166-CN | Mouse Anti-DEK Recombinant Antibody (HPAB-0166-CN) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0165-CN-S(P) | Mouse Anti-DEK Recombinant Antibody; scFv Fragment (HPAB-0165-CN-S(P)) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0166-CN-S(P) | Mouse Anti-DEK Recombinant Antibody; scFv Fragment (HPAB-0166-CN-S(P)) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0165-CN-F(E) | Mouse Anti-DEK Recombinant Antibody; Fab Fragment (HPAB-0165-CN-F(E)) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0166-CN-F(E) | Mouse Anti-DEK Recombinant Antibody; Fab Fragment (HPAB-0166-CN-F(E)) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0469-CN | Rabbit Anti-DEK Recombinant Antibody (clone JB36-32) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC450 | AbPlus™ Anti-DEK Magnetic Beads (VS-0724-YC450) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY654 | Anti-DEK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC297 | Rabbit Anti-DEK Recombinant Antibody (VS13-YC297) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1975 | Anti-Rat DEK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1974 | Anti-Human DEK Immunohistochemistry Kit | IHC |
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