Anti-ERG Antibody Prodrug, Protease Activated (VS-1025-YC48)
CAT#: VS-1025-YC48
The Anti-ERG Antibody Prodrug, Protease Activated (VS-1025-YC48) is an antibody prodrug comprised of a recombinant human antibody targeting human ERG, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to ERG protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to ERG effectively. ERG is a transcription factor that acts as a powerful oncogene when involved in chromosomal translocations, most notably the TMPRSS2-ERG gene fusion found in over 50% of prostate cancers. This fusion places the ERG gene under the control of the highly active TMPRSS2 promoter, leading to its massive overexpression and driving the malignant transformation and progression of the disease.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18377/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in tubules and cells in glomeruli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46598/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong positivity in cells of red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18377/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18377/40670_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18377/40670_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18377/40670_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18377/40670_A_8_5.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18377/40670_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (HUVEC TERT2, MOLT-4, REH, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157554-ERG
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human ERG
- Target
- ERG
- Species Reactivity
- Human
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The monoclonal antibodies that bind to ERG can be used to treat malignancies associated with overexpression of ERG or an ERG fusion event, such as prostate cancer, Ewing's sarcoma, acute myeloid leukemia, acute T-lymphoblastic leukemia, endothelial cancer, and colon cancer.
- Alternative Names
- ERG; v-ets avian erythroblastosis virus E26 oncogene homolog; p55; erg-3; transcriptional regulator ERG; ets-related; TMPRSS2/ERG fusion
- Gene ID
- 2078
- UniProt ID
- B4DN83
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-1025-YC48. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ERG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-332 | Recombinant Anti-human ERG Antibody | WB, Neut, FuncS | IgG |
| MHH-332 | Recombinant Human Anti-human ERG Antibody | ELISA, WB, Neut, FuncS | IgG |
| MRO-0542-CN | Rabbit Anti-ERG Recombinant Antibody (clone CBACN-214) | WB, IF, IHC, ChIP | Rabbit IgG |
| MRO-1843-CN | Rabbit Anti-ERG Polyclonal Antibody (MRO-1843-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ588 | Rabbit Anti-ERG Recombinant Antibody (VS3-CJ588) | WB, ICC, IF, IHC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-332-F(E) | Recombinant Anti-human ERG Antibody Fab Fragment | RIA, ELISA, FuncS | Fab |
| MHH-332-F(E) | Recombinant Human Anti-human ERG Antibody Fab Fragment | WB, IP, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-332-S(P) | Recombinant Anti-human ERG Antibody scFv Fragment | WB, IF, FuncS | scFv |
| MHH-332-S(P) | Recombinant Human Anti-human ERG Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-012WM | Mouse Anti-ERG Recombinant Antibody (TAB-012WM) | WB, ELISA, IP, IF | Mouse IgG |
| TAB-012WM-S(P) | Mouse Anti-ERG Recombinant Antibody; scFv Fragment (TAB-012WM-S(P)) | WB, ELISA, IP, IF | Mouse scFv |
| TAB-012WM-F(E) | Mouse Anti-ERG Recombinant Antibody; Fab Fragment (TAB-012WM-F(E)) | WB, ELISA, IP, IF | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1186 | Hi-Affi™ Rabbit Anti-ERG Recombinant Antibody (clone DS1186AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2347 | Anti-ERG Immunohistochemistry Kit | IHC | |
| VS-0525-XY2348 | Anti-Mouse ERG Immunohistochemistry Kit | IHC | |
| VS-0525-XY2349 | Anti-Rat ERG Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: Inhib, Cyt
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: Neut, ELISA, FuncS
Application: WB, ELISA, FC, IHC, IP
-5.jpg)
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
