AbPlus™ Anti-Gtf2f2 Magnetic Beads (VS-0724-YC159) (CAT#: VS-0724-YC159)
The AbPlus Anti-Gtf2f2 Magnetic Beads (VS-0724-YC159) is an innovative affinity resin which is bound with anti-Gtf2f2 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of Gtf2f2 protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/if_selected.jpg
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_B_7_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_8_3.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_7_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_7_5.jpg
Figure 6 Testis
(Round or early spermatids
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_6_6.jpg
(Round or early spermatids
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188342-GTF2F2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188342-GTF2F2
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-Gtf2f2 specific antibody
- Target
- Gtf2f2
- Immunogen
- Recombinant human Gtf2f2 fragment derived from E. coli.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription preinitiation complex.
- Alternative Names
- BTF4; RAP30; TF2F2; TFIIF
- Gene ID
- 2963
- UniProt ID
- P13984
Downloads
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