AbPlus™ Anti-ISG15 Magnetic Beads (VS-0724-YC800)
CAT#: VS-0724-YC800
The AbPlus Anti-ISG15 Magnetic Beads (VS-0724-YC800) is an innovative affinity resin which is bound with anti-ISG15 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of ISG15 protein under native and denaturing conditions.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, HeLa, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187608-ISG15
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-ISG15 specific antibody
- Target
- ISG15
- Immunogen
- Recombinant human ISG15, aa 1-165.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]
- Alternative Names
- ISG15 Ubiquitin-Like Modifier
- Gene ID
- 9636
- UniProt ID
- P05161
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Vital For Antiviral Studies
ISG15 is a key player in our viral infection studies, and these beads allowed us to easily isolate ISGylated viral proteins. The enrichment factor was high, enabling us to detect modifications that were crucial for understanding the host antiviral response.
Clean And Quick Results
The protocol for these beads is very efficient. We obtained clean results with minimal non-specific binding, which helped us clearly define the ISG15 interactome in our stimulated macrophage cell lines without extensive optimization steps.
Q&As
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What biological process can be studied using these ISG15 beads?
A: These beads are ideal for studying ISGylation, a ubiquitin-like modification where the ISG15 protein is conjugated to target proteins. This process is highly upregulated during viral infections as part of the innate immune response, so the beads are essential for identifying viral and host proteins targeted by ISG15.
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How do these beads assist in viral host interaction research?
A: By using these beads to immunoprecipitate ISGylated proteins from infected cells, researchers can map the "ISGylome." This helps in understanding how the host cell uses ISG15 to interfere with viral replication or how viruses might evolve mechanisms to evade this immune defense.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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