AbPlus™ Anti-PHPT1 Magnetic Beads (VS-0724-YC940) (CAT#: VS-0724-YC940)
The AbPlus Anti-PHPT1 Magnetic Beads (VS-0724-YC940) is an innovative affinity resin which is bound with anti-PHPT1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of PHPT1 protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20952/188_E3_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20952/188_E3_2_selected.jpg

Figure 2 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity of mucus in the goblet cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20952/ihc_selected.jpg
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity of mucus in the goblet cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20952/ihc_selected.jpg

Figure 3 IHC staining of human vulva/anal skin
(Immunohistochemical staining of human vulva/anal skin shows distinct cytoplasmic positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/ihc_selected.jpg
(Immunohistochemical staining of human vulva/anal skin shows distinct cytoplasmic positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/ihc_selected.jpg

Figure 4 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_B_8_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_B_8_5.jpg

Figure 5 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_8_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_8_4.jpg

Figure 6 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_7_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_7_5.jpg

Figure 7 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_6_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_6_6.jpg

Figure 8 Lymph node
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_9_8.jpg
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_9_8.jpg

Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000054148-PHPT1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000054148-PHPT1
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-PHPT1 specific antibody
- Target
- PHPT1
- Immunogen
- Recombinant human PHPT1, aa 2-125.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
- Alternative Names
- Phosphohistidine Phosphatase 1; Sex-Regulated Protein Janus-A; Protein Histidine Phosphatase; Protein Janus-A Homolog; PHP14; PHP; Epididymis Secretory Sperm Binding Protein Li 132P; 14 KDa Phosphohistidine Phosphatase;
- Gene ID
- 29085
- UniProt ID
- Q9NRX4
Downloads
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-774 | Recombinant Anti-human PHPT1 Antibody | WB, IHC, FuncS | IgG |
MHH-774 | Recombinant Human Anti-human PHPT1 Antibody | WB, FC, FuncS | IgG |
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