AbPlus™ Anti-PHPT1 Magnetic Beads (VS-0724-YC940)
CAT#: VS-0724-YC940
The AbPlus Anti-PHPT1 Magnetic Beads (VS-0724-YC940) is an innovative affinity resin which is bound with anti-PHPT1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of PHPT1 protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20952/188_E3_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity of mucus in the goblet cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20952/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human vulva/anal skin
Immunohistochemical staining of human vulva/anal skin shows distinct cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_B_8_5.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13584/28573_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000054148-PHPT1
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-PHPT1 specific antibody
- Target
- PHPT1
- Immunogen
- Recombinant human PHPT1, aa 2-125.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
- Alternative Names
- Phosphohistidine Phosphatase 1; Sex-Regulated Protein Janus-A; Protein Histidine Phosphatase; Protein Janus-A Homolog; PHP14; PHP; Epididymis Secretory Sperm Binding Protein Li 132P; 14 KDa Phosphohistidine Phosphatase;
- Gene ID
- 29085
- UniProt ID
- Q9NRX4
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PHPT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-774 | Recombinant Anti-human PHPT1 Antibody | WB, IHC, FuncS | IgG |
| MHH-774 | Recombinant Human Anti-human PHPT1 Antibody | WB, FC, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-774-F(E) | Recombinant Anti-human PHPT1 Antibody Fab Fragment | RIA, IF, FuncS | Fab |
| MHH-774-F(E) | Recombinant Human Anti-human PHPT1 Antibody Fab Fragment | ELISA, FacS, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-774-S(P) | Recombinant Anti-human PHPT1 Antibody scFv Fragment | FC, IP, FuncS | scFv |
| MHH-774-S(P) | Recombinant Human Anti-human PHPT1 Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4363 | Hi-Affi™ Recombinant Rabbit Anti-PHPT1 Monoclonal Antibody (SI453DS) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5381 | Anti-PHPT1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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