AbPlus™ Anti-RAB6A Magnetic Beads (VS-0724-YC840)
CAT#: VS-0724-YC840
The AbPlus Anti-RAB6A Magnetic Beads (VS-0724-YC840) is an innovative affinity resin which is bound with anti-RAB6A specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of RAB6A protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59131/1301_C10_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity, with a dot like pattern in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59131/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9936/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59131/1240_F12_4_blue_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59131/994_C10_4_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59131/134123_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59131/134123_A_9_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59131/134123_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59131/134123_A_5_6.jpg
Normal Tissue
Figure 10 Placenta
Decidual cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59131/134123_A_1_7.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000175582-RAB6A
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-RAB6A specific antibody
- Target
- RAB6A
- Immunogen
- Recombinant human Rab6/Rab GTPase/RAB6A, aa 1-208.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes a member of the RAB family, which belongs to the small GTPase superfamily. GTPases of the RAB family bind to various effectors to regulate the targeting and fusion of transport carriers to acceptor compartments. This protein is located at the Golgi apparatus, which regulates trafficking in both a retrograde (from early endosomes and Golgi to the endoplasmic reticulum) and an anterograde (from the Golgi to the plasma membrane) directions. Myosin II is an effector of this protein in these processes. This protein is also involved in assembly of human cytomegalovirus (HCMV) by interacting with the cellular protein Bicaudal D1, which interacts with the HCMV virion tegument protein, pp150. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
- Alternative Names
- RAB6A, Member RAS Oncogene Family; RAB6; RAB6, Member RAS Oncogene Family; Ras-Related Protein Rab-6A; Rab GTPase; Rab-6;
- Gene ID
- 5870
- UniProt ID
- P20340
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| MOB-1192CT | Recombinant Mouse anti-Human RAB6A Monoclonal antibody (EML668) | ICC, IF, IHC-P, WB |
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| VS-0525-XY5933 | Anti-RAB6A Immunohistochemistry Kit | IHC | |
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