AbPlus™ Anti-RPS3 Magnetic Beads (VS-0724-YC387)
CAT#: VS-0724-YC387
The AbPlus Anti-RPS3 Magnetic Beads (VS-0724-YC387) is an innovative affinity resin which is bound with anti-RPS3 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of RPS3 protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63339/1230_E7_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skin
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63339/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63339/142624_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63339/142624_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63339/142624_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63339/142624_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63339/142624_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63339/142624_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149273-RPS3
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-RPS3 specific antibody
- Target
- RPS3
- Immunogen
- Synthetic peptide corresponding to the C-terminus of the human RPS3.
- Target Species
- Human, Mouse, Rat
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
- Alternative Names
- Ribosomal Protein S3; Small Ribosomal Subunit Protein US3; IMR-90 Ribosomal Protein S3; 40S Ribosomal Protein S3; EC 4.2.99.18; S3;
- Gene ID
- 6188
- UniProt ID
- P23396
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC438 | AbPlus™ Anti-RPS3 Magnetic Beads (VS-0724-YC438) | IP, Protein Purification |
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| VS-0325-XY1963 | Anti-RPS3 Immunohistochemistry Kit | IHC | |
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