Human Anti-Tetanus toxin Recombinant Antibody (clone 243-10) (CAT#: FAMAB-0700WJ)

Figure 1 Monoclonal antibodies reactivity by ELISA using 96-wells plates

Monoclonal antibodies reactivity by ELISA using 96-wells plates coated with (A) 5 μg/mL tetanus toxin, (B) 5 μg/mL tetanus toxoid, and (C) 2 μg/mL recombinant tetanus toxin fragment C. (D) mAbs capacity to inhibit TeNT binding was assessed by incubating GT1b with an appropriate level of TeNT alone or with increasing concentrations of each mAb. The mAbs were tested in the range from 0.4 to 50 ng/mL. Serum of a vaccinated person was used as positive control for A-C; horse hyperimmune serum was used in assay D.

Aliprandini, E., Takata, D. Y., Lepique, A., Kalil, J., Boscardin, S. B., & Moro, A. M. (2019). An oligoclonal combination of human monoclonal antibodies able to neutralize tetanus toxin in vivo. Toxicon: X, 2, 100006.

Figure 2 Western Blotting showing the recognition of different TeNT chains by the mAbs.

In results of WB: BUT-TT-140-08 e BUT-TT-117-08 recognized the heavy chain (b); BUT-TT-120-10 recognized the light chain (c) and the entire TeNT (a), which was expected since it also contains the light fragment; BUT-TT-243-10 recognized the heavy chain (b) and the entire TeNT (a); and BUT-TT-143-10 recognized only the non-processed (entire) TeNT.

Aliprandini, E., Takata, D. Y., Lepique, A., Kalil, J., Boscardin, S. B., & Moro, A. M. (2019). An oligoclonal combination of human monoclonal antibodies able to neutralize tetanus toxin in vivo. Toxicon: X, 2, 100006.

Figure 3 In vivo tetanus toxin neutralizing assay.

The in vivo neutralizing capacity of TeNT-specific mAbs was evaluated using Swiss mice in accordance to the potency test used for approval of hyperimmune horse sera. A fixed amount of reference TeNT was incubated with the serially diluted mAbs (alone or mixed) and injected subcutaneously into 10 mice for each dilution. The values indicated in the legend correspond to the amount of mAb injected in each one of 10 animals. After four days of observation for any signs of tetanus the survival rates were calculated according to a probit statistical model.

Aliprandini, E., Takata, D. Y., Lepique, A., Kalil, J., Boscardin, S. B., & Moro, A. M. (2019). An oligoclonal combination of human monoclonal antibodies able to neutralize tetanus toxin in vivo. Toxicon: X, 2, 100006.