Mouse Anti-TNNI3 Recombinant Antibody (clone K2b5) (CAT#: VS3-WK702)
This product is a mouse monoclonal antibody that recognizes TNNI3.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1153_D2_2_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1153_D2_2_selected.jpg
Figure 2 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1220_C7_6_blue_red_green.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1220_C7_6_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1118_D2_2_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1118_D2_2_blue_red_green.jpg
Figure 4 Testis
(Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_A_6_6.jpg
(Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_A_6_6.jpg
Figure 5 Heart muscle
(Cardiomyocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_B_4_6.jpg
(Cardiomyocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_B_4_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (HEK93, HMC-1, K-562, OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129991-TNNI3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129991-TNNI3
Specifications
- Immunogen
- Native cTnI
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TNNI3
- Species Reactivity
- Human
- Clone
- K2b5
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >98% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Storage
- Store at -20°C for 12 months. Avoid repeated freezing and thawing.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot and ELISA.
Target
- Alternative Names
- CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
- Gene ID
- 7137
- UniProt ID
- P19429
- Long Name
- Troponin I3, cardiac type
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1096 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment | ELISA, WB, IHC, IP | Human scFv |
| DrMAB-1099 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment, HRP | ELISA, WB, IHC, IP | Human scFv |
| FAMAB-0154-CN-S(P) | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4); scFv Fragment | ELISA | Rabbit scFv |
| HPAB-0942-FY-F(E) | Mouse Anti-TNNI3 Recombinant Antibody Fab Fragment (HPAB-0942-FY-F(E)) | FuncS, ELISA | Mouse Fab |
| HPAB-0497-YJ-S(P) | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ); scFv Fragment | ELISA | Mouse scFv |
Customer Reviews and Q&As
Customer Review
Q&As
Unmatched clarity and specificity
The K2b5 antibody has altered our Western blotting results. The clarity and specificity of the bands are unparalleled, saving us time and money on repeat studies.
Sensitive and specific
For ELISA assays detecting TNNI3, the K2b5 antibody is incredibly sensitive and specific. It's our go-to reagent for quantifying this crucial cardiac marker.
Highly recommend for IP
Using the K2b5 clone for immunoprecipitation has given us clean and specific pull-downs of TNNI3, a significant improvement over our previous antibodies. Highly recommend.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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