Mouse Anti-TNNI3 Recombinant Antibody (clone K2b5)
CAT#: VS3-WK702
This product is a mouse monoclonal antibody that recognizes TNNI3.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1153_D2_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1220_C7_6_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/1118_D2_2_blue_red_green.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_A_6_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46428/142463_B_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEK93, HMC-1, K-562, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129991-TNNI3
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Specifications
- Immunogen
- Native cTnI
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TNNI3
- Species Reactivity
- Human
- Clone
- K2b5
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >98% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Storage
- Store at -20°C for 12 months. Avoid repeated freezing and thawing.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot and ELISA.
Target
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Unmatched clarity and specificity
The K2b5 antibody has altered our Western blotting results. The clarity and specificity of the bands are unparalleled, saving us time and money on repeat studies.
Sensitive and specific
For ELISA assays detecting TNNI3, the K2b5 antibody is incredibly sensitive and specific. It's our go-to reagent for quantifying this crucial cardiac marker.
Highly recommend for IP
Using the K2b5 clone for immunoprecipitation has given us clean and specific pull-downs of TNNI3, a significant improvement over our previous antibodies. Highly recommend.
Q&As
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What is the binding affinity of Mouse Anti-TNNI3 Recombinant Antibody (clone K2b5) for the target antigen?
A: The Mouse Anti-TNNI3 Recombinant Antibody (clone K2b5) has a high binding affinity, typically in the low nanomolar range. This strong binding ensures specific and reliable detection of Troponin I type 3, cardiac (TNNI3), in various assays.
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Is the Recombinant Mouse Anti-TNNI3 Antibody (clone K2b5) applicable for Western blot analysis?
A: Yes, the K2b5 antibody has been successfully validated for use in Western blotting. It provides clear and specific bands for the TNNI3 protein, making it an excellent choice for this application.
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What is the recommended storage condition for maintaining the stability of the Mouse Anti-TNNI3 Recombinant Antibody (clone K2b5)?
A: The K2b5 antibody should be stored at -20°C for long-term storage. For short-term use, it can be kept at 4°C. Avoid repeated freeze-thaw cycles to maintain its stability and binding efficacy.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "TNNI3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1096 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment | ELISA, WB, IHC, IP | Human scFv |
| DrMAB-1099 | Human Anti-TNNI3 Recombinant Antibody (clone 1542.14A2); scFv Fragment, HRP | ELISA, WB, IHC, IP | Human scFv |
| FAMAB-0154-CN-S(P) | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4); scFv Fragment | ELISA | Rabbit scFv |
| HPAB-0942-FY-F(E) | Mouse Anti-TNNI3 Recombinant Antibody Fab Fragment (HPAB-0942-FY-F(E)) | FuncS, ELISA | Mouse Fab |
| HPAB-0497-YJ-S(P) | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-733 | Chicken Anti-TNNI3 Recombinant Antibody (clone mAb180) | WB, IHC, IF, FuncS | Chicken IgG |
| FAMAB-0154-CN | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4) | ELISA | Rabbit IgG |
| MRO-0232-CN | Rabbit Anti-TNNI3 Recombinant Antibody (clone JF0550) | WB, IF, IHC, IP | Rabbit IgG |
| FAMAB-1278CQ | Human Anti-TNNI3 Recombinant Antibody (clone scFv 180) | ELISA, WB | Human IgG1 |
| ZG-0143U | Mouse Anti-TNNI3 Recombinant Antibody (clone 11D2E10) | ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2209CQ | Mouse Anti-TNNI3 Recombinant Antibody (NEUT-2209CQ) | ELISA, Inhib, WB | Mouse IgG1 |
| NEUT-2210CQ | Mouse Anti-TNNI3 Recombinant Antibody (VMC208) | ELISA, Inhib, WB | Mouse IgG2b |
| NEUT-2211CQ | Mouse Anti-TNNI3 Recombinant Antibody (clone 4C2) | ELISA, Inhib, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3627 | Rabbit Anti-TNNI3 Recombinant Antibody (clone DS3627AB) | ICC, IF, IHC-Fr, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0154-CN-F(E) | Rabbit Anti-TNNI3 Recombinant Antibody (clone MG4); Fab Fragment | ELISA | Rabbit Fab |
| HPAB-0942-FY-S(P) | Mouse Anti-TNNI3 Recombinant Antibody scFv Fragment (HPAB-0942-FY-S(P)) | FuncS, ELISA | Mouse scFv |
| HPAB-0497-YJ-F(E) | Mouse Anti-TNNI3 Recombinant Antibody (clone CBT62YJ); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY260 | AbPlus™ Anti-TNNI3 Magnetic Beads (1542.14A2) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2324 | Anti-TNNI3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7407 | Anti-Mouse TNNI3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7406 | Anti-Human TNNI3 Immunohistochemistry Kit | IHC |
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