AbPlus™ Anti-TUBA1A Magnetic Beads (8F11)
CAT#: VS-0424-XY266
The AbPlus Anti-TUBA1A Magnetic Beads (8F11) is an innovative affinity resin which is bound with anti-TUBA1A specific antibody (8F11). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of TUBA1A protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39247/569_B12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43684/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39247/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39247/563_B12_1_blue_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8686/23063_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, SCLC-21H)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167552-TUBA1A
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-TUBA1A specific antibody (8F11)
- Target
- TUBA1A
- Immunogen
- Recombinant protein of tubulin alpha-1A chain
- Target Species
- Human, Rat, Mouse
- Antibody Clone
- 8F11
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
- Alternative Names
- B-ALPHA-1; LIS3; TUBA3
- Gene ID
- 7846
- UniProt ID
- Q71U36
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "TUBA1A"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1002 | Human Anti-TUBA1A Recombinant Antibody (clone F2C), Atto 488 | ICC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1073 | Human Anti-TUBA1A Recombinant Antibody (clone F2C) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
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| MOB-1097MZ | Recombinant Mouse Anti-Mouse TUBA1A Antibody (clone BB23) | ICC, IF | Mouse antibody |
| CAT | Product Name | Application | Type |
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| MRO-0203YJ | Rabbit Anti-TUBA1A Polyclonal Antibody | WB, IHC, IF, ICC, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| ZG-0186C | Mouse Anti-TUBA1A Recombinant Antibody (ZG-0186C) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
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| VS-0325-XY2391 | Anti-TUBA1A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7600 | Anti-Mouse TUBA1A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY7599 | Anti-Human TUBA1A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7601 | Anti-Rat TUBA1A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY7602 | Anti-Monkey TUBA1A Immunohistochemistry Kit | IHC |
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