AbPlus™ Anti-UBE4A Magnetic Beads (VS-0724-YC954) (CAT#: VS-0724-YC954)
The AbPlus Anti-UBE4A Magnetic Beads (VS-0724-YC954) is an innovative affinity resin which is bound with anti-UBE4A specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of UBE4A protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55035/895_E2_5_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55035/895_E2_5_selected.jpg

Figure 2 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64010/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64010/if_selected.jpg

Figure 3 Small intestine
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55035/128434_A_4_2.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55035/128434_A_4_2.jpg

Figure 4 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55035/128434_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55035/128434_A_6_6.jpg

Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110344-UBE4A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110344-UBE4A
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-UBE4A specific antibody
- Target
- UBE4A
- Immunogen
- Synthetic peptide corresponding to the N-terminus of the human UBE4A.
- Target Species
- Human, Rat
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]
- Alternative Names
- Ubiquitination Factor E4A; Ubiquitination Factor E4A (UFD2 Homolog, Yeast); RING-Type E3 Ubiquitin Transferase E4 A; Ubiquitination Factor E4A (Homologous To Yeast UFD2); Ubiquitin Conjugation Factor E4 A; EC 2.3.2.27;
- Gene ID
- 9354
- UniProt ID
- Q14139
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2128CT | Recombinant Mouse anti-Human UBE4A Monoclonal antibody (EML914) | WB |
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