The enzyme is a protease that hydrolyzes mucin-type O-glycans (including sialic acids). It is recommended to incubate the samples at 37 °C for 2 hours. It can not digest unmodified serine (Ser, S) and threonine (Thr,T) residues, or glycoproteins with only N-linked glycans. This protease was cloned from P. aeruginosa and produced in E. coli with His-tag for easy purification.
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