Quick™ Europium Antibody Labeling Kit (VS-0824-FY99)
CAT#: VS-0824-FY99
The europium antibody labeling kit offers a quick and efficient method for conjugating antibodies or proteins to europium chelate microspheres. The specially treated surface of the particles allows for easy attachment of biomolecules via lysine residues, resulting in stable and sensitive conjugates. The kit includes all necessary buffers and reagents for conjugation, and the process can be completed in just 35 minutes with minimal hands-on time. The particles are resistant to aggregation and provide a 15-fold higher sensitivity in immunoassays compared to other common particles. Additionally, the conjugation procedure is not highly dependent on the isoelectric point of the antibody, eliminating the need for extensive pH testing. The europium antibody labeling kit is fully scalable for both research and manufacturing purposes, making it a valuable tool for immunoassay development.
Specifications
- Fluorescent Dye Name
- Europium
- Application
- Dolt Blot
- Composition
- 200 nm europium vial (lyophilized)
Europium quencher (10X)
Reaction buffer A (1X)
Reaction buffer B (1X)
Resuspension buffer (1X)
- Procedure Steps
- 1. Ensure all reagents are at room temperature before proceeding.
2. Prepare a 0.1 mg/mL dilution of the stock antibody with either Reaction buffer A or B. Use 45 μL of this diluted antibody to conjugate one 200 nm Europium vial.
Note: For optimal results, it is recommended to do the reaction twice with each buffer and also try different dilutions of the antibody.
3. Add 40 μL of the 0.1 mg/mL antibody solution to the 200 nm europium vial and mix thoroughly by gently pipetting up and down. Incubate the reaction at room temperature for exactly 15 minutes.
4. Dilute the 10x europium quencher with deionized water. Prepare 1.2 mL of 1x europium quencher for each vial by mixing 120 μL of the 10x quencher with 1080 μL water.
5. After 15 minutes, add 1 mL of the 1x europium quencher to stop the reaction and mix by gently inverting the vial.
6. Allow the reaction to quench for 5 minutes at room temperature. Transfer the contents into a microcentrifuge tube and centrifuge at 13800 x g for 8 minutes. Remove approximately 850 μL of the supernatant and then spin for an additional 2 minutes to remove the remaining supernatant.
7. Tap the pellet gently and add 40 μL of resuspension buffer. Thoroughly resuspend the pellet by pipetting up and down for at least 90 seconds.
8. The resulting solution is now 40 μL of 1% conjugate.
9. Dilute the conjugate to the desired concentration for your specific application.
Note: It is recommended to dilute the conjugate to 0.0025%-0.005%, but further optimization may be necessary depending on your particular needs.
Please refer to the COA of the shipped product.
- Product Precautions
- It is suggested to have the antibody at a concentration of 1 mg/mL in a buffer such as 10-50 mM MES, HEPES, or MOPS at pH 6-7 (without any additional components like salt) before conjugation. For more information on incompatible buffers, please consult the protocol booklet.
This product is intended for research purposes only and is not intended for use in human, therapeutic, or diagnostic applications.
- Kit Storage
- Upon receipt, store the kit at -20°C immediately. The buffers and the quencher can be stored at either +4°C or -20°C. The kit can be stored for up to 1 year from receipt if the components have not been reconstituted.
- Conjugate Storage
- It is recommended to store the conjugate at 4°C, using the Resuspension Buffer from the conjugation reaction as a storage buffer. Avoid storing the conjugate at -20°C, as this may affect its stability. The stability of the conjugate is primarily dependent on the antibody used, so as long as the antibody remains stable, the conjugate should also remain stable.
- Sample Type
- Antibody sample
- Reactive Species
- The reactive species of the kit depends on the research application of the antibody.
- Fluorescent Dye Introduction
- Europium conjugation refers to the process of attaching europium ions to molecules or particles in order to create conjugates for use in various biological and analytical applications. Europium is a rare earth element that emits a strong and stable fluorescence when excited by ultraviolet light, making it a popular choice for labeling biomolecules in fluorescence assays.
Overall, europium conjugation is a powerful technique that enables the development of innovative and reliable tools for a wide range of biological and analytical applications.
- Alter Name
- Quick™ Europium Antibody Labeling Kit; Europium Antibody Labeling Kit
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