Fab Fragment Preparation Kit (VS-0924-WK1)
CAT#: VS-0924-WK1
This Fab preparation kit uses immobilized papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose. It is suitable for human, rabbit, mouse, and other species and subclasses of IgG. The papain antibody digestion reaction is performed in convenient disposable spin columns that allow efficient removal of the immobilized protease and maximum recovery of the IgG fragments.
Specifications
- Product Size
- 10 Antibody Samples, each containing 0.25 to 4 mg IgG
- Format
- Kit
- Contents
- Papain agarose, 1.25 mL
Cysteine-HCl, 1 g
Fab digestion buffer, 120 mL
Protein A spin column, 1 mL, 1 column
PBS packs (each makes 500 mL), 2 packs
IgG elution buffer, 120 mL
Desalt spin columns, 7K MWCO, 2mL, 10 columns
Spin columns, 0.8 mL, 10 columns
Microcentrifuge tubes, 2 mL, 30 tubes
- Specifications
- Sufficient for: 10 antibody samples, each containing 0.25 to 4 mg IgG.
- Storage
- Store at 4-8°C.
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Simple Fab Preparation
This kit made the generation of Fab fragments incredibly straightforward. The protocol was easy to follow, and the included buffers were optimized perfectly, resulting in a high yield of Fab fragments with very little undigested IgG remaining in the final product.
Reduced Background In IHC
We used the Fab fragments generated by this kit for our immunohistochemistry staining. By eliminating the Fc portion, we significantly reduced the non-specific background signal caused by endogenous Fc receptors in the tissue samples, making our images much clearer.
Q&As
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What is the main purpose of this Fab Fragment Preparation Kit?
A: This kit is designed to prepare Fab fragments from IgG antibodies. It utilizes specific enzymes to cleave the antibody, removing the Fc region and leaving the antigen-binding Fab portion. This is useful for applications where the Fc region might cause non-specific binding or interference.
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Does the kit include everything needed for the digestion and purification?
A: Yes, the kit comes with optimized buffers and purification columns to facilitate the entire process. It simplifies the workflow by providing pre-optimized conditions for the enzymatic digestion and subsequent purification, ensuring you can obtain high-quality Fab fragments with minimal optimization effort.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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