Fab Fragment Preparation Kit (VS-0924-WK1)
CAT#: VS-0924-WK1
This Fab preparation kit uses immobilized papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose. It is suitable for human, rabbit, mouse, and other species and subclasses of IgG. The papain antibody digestion reaction is performed in convenient disposable spin columns that allow efficient removal of the immobilized protease and maximum recovery of the IgG fragments.
Specifications
- Product Size
- 10 Antibody Samples, each containing 0.25 to 4 mg IgG
- Format
- Kit
- Contents
- Papain agarose, 1.25 mL
Cysteine-HCl, 1 g
Fab digestion buffer, 120 mL
Protein A spin column, 1 mL, 1 column
PBS packs (each makes 500 mL), 2 packs
IgG elution buffer, 120 mL
Desalt spin columns, 7K MWCO, 2mL, 10 columns
Spin columns, 0.8 mL, 10 columns
Microcentrifuge tubes, 2 mL, 30 tubes
- Specifications
- Sufficient for: 10 antibody samples, each containing 0.25 to 4 mg IgG.
- Storage
- Store at 4-8°C.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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