Quick™ Latex Antibody Labeling Kit (VS-0824-FY130) (CAT#: VS-0824-FY130)
Latex conjugation or labeling can be effectively accomplished in < four hours, requiring only 30 seconds of active hands-on time when utilizing the latex antibody Labeling kit-400 nm black or the latex antibody Labeling kit-400 nm red. This process ensures 100% recovery of antibodies.
| Sub Cat | Fluorescent Dye Name | Product Name | |
| VS-0824-FY85 | Latex-400 nm Black | Quick™ Latex Antibody Labeling Kit, 400 nm Black | |
| VS-0824-FY86 | Latex-400 nm Red | Quick™ Latex Antibody Labeling Kit, 400 nm Red |
More Infomation
Specifications
- Fluorescent Dye Name
- Latex-400 nm
- Composition
- 400 nm black or red latex vial (Lyophilized)
Quencher (10X)
Reaction buffer A (1X)
Reaction buffer B (1X)
Resuspension buffer
- Procedure Steps
- 1. Allow all reagents to warm to room temperature.
2. Dilute the stock antibody to 0.1 mg/mL with reaction buffer A or B. 45 μL is sufficient to bind one 400 nm black latex vial or red latex vial.
3. Add 40 μL of 0.1 mg/mL antibody to the 400 nm black latex vial or red latex vial and reconstitute the latex by gently pipetting up and down thoroughly. Incubate the reaction at room temperature for 15 minutes.
4. Dilute enough 10x quencher with deionized water. For one 400 nm black latex vial, you will need exactly 1 mL, so we recommend that you prepare 1.2 mL of 1x quencher per vial, which is 120 μL 10x quencher + 1080 μL water.
5. After 15 minutes, add 1 mL 1X quencher to stop the reaction and mix by inverting several times.
6. Allow the reaction to quench at room temperature for 5 minutes, then transfer to a microcentrifuge tube and spin at 10,000 rpm for 9 minutes. Remove approximately 850 μL of supernatant and without resuspending the pellet, spin at 10,000 rpm for another 1 minute. Remove the remaining supernatant.
7. Gently resuspend the pellet in 40 μL of Resuspension buffer or the storage buffer of your choice.
8. You now have 40 μL of 1% conjugate. Dilute the conjugate as needed for your application.
Please refer to the COA of the shipped product.
- Product Precautions
- We recommend that the antibody to be labeled should be in a solution of 10-50 mM MES, HEPES, or MOPS at pH 6-7 (without any additional components such as salt) prior to conjugation.
This product is intended for research purposes only and is not intended for use in human, therapeutic, or diagnostic applications.
- Kit Storage
- Store kit at -20°C immediately upon receipt.
- Conjugate Storage
- It is recommended to store any conjugate at 4°C. The resuspension buffer added at the end of the conjugation reaction is a good storage buffer. Do not store conjugates at -20°C. The determining factor for conjugate stability is the antibody itself, as it will degrade first. Therefore, if your antibody is stable, the conjugate will also be stable.
- Assay Platform
- The choice of detection method often depends on the desired application and specific experimental purposes. Common detection methods include spectroscopic detection (such as absorbance measurement), fluorescence detection (such as fluorescence microscopy or flow cytometry), and electrochemical detection (such as electrochemical methods).
- Sample Type
- Antibody sample
- Reactive Species
- The reactive species of the kit depends on the research application of the antibody.
- Fluorescent Dye Introduction
- Latex Antibody Labeling Kits are essential tools used in bioconjugation techniques for attaching molecules of interest to latex particles. This process allows for the detection and quantification of target molecules in various biological samples. This kit typically includes latex beads with functional groups for conjugation, as well as all necessary buffers and reagents for successful coupling. Latex Antibody Labeling Kits are widely used in research labs, diagnostics, and drug development applications for a variety of assays, including immunoassays, flow cytometry, and magnetic separation.
- Alter Name
- Quick™ Latex Antibody Labeling Kit, 400 nm Black; Quick™ Latex Antibody Labeling Kit, 400 nm Red
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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