Quick™ mFluor Violet 510 Antibody Labeling Kit (VS-0824-FY96)
CAT#: VS-0824-FY96
The mFluor Violet 510 antibody labeling kit is a convenient and efficient method for labelling small amounts of proteins with the Violet Laser-excitable mFluor Violet 510 dye. It is easy to use and provides stable and specific labelling results.
Specifications
- Fluorescent Dye Name
- mFluor Violet 510
- Application
- Flow Cytometry
- Composition
- Reaction buffer
TQ-Dyed quench buffer
mFluor Violet 510
- Procedure Steps
- Start conjugation reaction
1. Combine the protein working solution (mFluor Violet 510) with one vial of labeling dye. Mix thoroughly by pipetting or vortexing the vial.
Note: If labeling 100 µg of protein, divide the protein into 2 x 50 µg portions and react each portion with one vial of labeling dye before combining both vials for the next step.
2. Allow the conjugation reaction mixture to sit at room temperature for 30-60 minutes.
Note: The mixture can be rotated or shaken for a longer duration if desired.
Stop conjugation reaction
1. Add 5 µL (for 50 µg protein) or 10 µL (for 100 µg protein) of TQ-Dyed quench buffer (10% of the total reaction volume) to the conjugation reaction mixture. Mix well.
2. Incubate at room temperature for 10 minutes. The labeled protein (antibody) is now ready for use.
Please refer to the COA of the shipped product.
- Product Precautions
- This product is intended for research purposes only and is not intended for use in human, therapeutic, or diagnostic applications.
- Kit Storage
- Upon receiving, store the product at -20°C.
- Conjugate Storage
- To maintain stability, store the protein conjugate at a concentration of> 0.5 mg/mL with a carrier protein such as 0.1% bovine serum albumin. For extended storage, consider lyophilizing the protein conjugates or dividing them into single-use aliquots and storing them at ≤ -20°C.
- Sample Type
- Antibody sample
- Reactive Species
- The reactive species of the kit depends on the research application of the antibody.
- Fluorescent Dye Introduction
- mFluor Violet 510 is a fluorescent dye used for cell labeling and flow cytometry applications. It emits a violet light with a peak wavelength of 510 nm when excited by the appropriate light source. mFluor Violet 510 is known for its high brightness and photostability, making it ideal for long-term imaging and tracking of cells in biological samples. It is commonly used in multiplexing experiments due to its compatibility with other fluorophores. This dye is water soluble and has minimal cytotoxicity, allowing for reliable staining of cells without affecting their viability or function. Overall, mFluor Violet 510 is a versatile fluorescent label with excellent performance characteristics for a variety of experimental applications.
- Alter Name
- Quick™ mFluor Violet 510 Antibody Labeling Kit; mFluor Violet 510 Antibody Labeling Kit
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Downloadable Resources
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Datasheet
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COA
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