Mouse IgG1 Fab and F(ab')2 Fragment Preparation Kit (VS-0924-WK3)
CAT#: VS-0924-WK3
This mouse IgG1 Fab and F(ab')2 preparation kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions. It contains sufficient reagents to generate and purify Fab and F(ab´)2 fragments from ten 0.5 mL samples containing 0.25-4mg IgG. Ficin generates F(ab´)2 fragments exclusively in the presence of 1-4 mM cysteine; Fab fragments are generated in the presence of 25 mM cysteine.
Specifications
- Product Size
- 2.5 mL
- Format
- Kit
- Contents
- Immobilized ficin, 2.5 mL settled resin, support is 6 % crosslinked beaded agarose supplied as 33 % slurry
Cysteine•HCl•H2O, 1 g
Mouse IgG1 digestion buffer, 120 mL, pH 6.0
Protein A spin column, 1 mL, 1 each
Protein A binding buffer, 120 mL
IgG elution buffer, 120 mL, pH 2.8, contains primary amine
Spin columns, 10 each, 0.8 mL spin columns with 10 top caps and 11 bottom plugs
Microcentrifuge tubes, 30 each, 2.0 mL spin column collection tubes
Spin desalting columns, 2 mL, 10 each, for 200-700 µL samples
- Specifications
- Sufficient for: 10 antibody samples, each containing 0.25 to 4 mg IgG.
- Storage
- Store at 4-8°C.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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