Mouse IgG1 Fab and F(ab')2 Fragment Preparation Kit (VS-0924-WK3) (CAT#: VS-0924-WK3)

This mouse IgG1 Fab and F(ab')2 preparation kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions. It contains sufficient reagents to generate and purify Fab and F(ab´)2 fragments from ten 0.5 mL samples containing 0.25-4mg IgG. Ficin generates F(ab´)2 fragments exclusively in the presence of 1-4 mM cysteine; Fab fragments are generated in the presence of 25 mM cysteine.