Mouse Immunoglobulin Isotyping ELISA Kit (VS-0924-WK4)
CAT#: VS-0924-WK4
The mouse antibody ELISA kit contains pre-matched antibody pairs, plates and reagents for performing ELISA to detect and identify mouse Igs.
Specifications
- Product Size
- 10 x 96 Tests
- Format
- Kit
- Contents
- Capture antibody: pre-titrated, purified antibody
Detection antibody: pre-titrated, HRP-conjugated antibody
Standard: recombinant protein for generating standard curve and calibrating samples
10X coating buffer: buffer for plating the capture antibody
5X ELISA diluent: buffer for blocking and diluting the detection antibody and enzyme
Substrate: 1X TMB solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96-well plate
- Specifications
- Target specificity: Mouse Ig kappa light chain, Ig lambda light chain, IgA, IgG1, IgG2a, IgG2b, IgG3, IgM
- Storage
- 2-8°C
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Quick Isotype Identification
This kit saved us a lot of guesswork. We had several new hybridoma clones, and this ELISA kit allowed us to quickly and accurately identify the isotype of each secreted antibody. The results were clear and easy to interpret within just a few hours.
Easy To Use Protocol
The protocol provided was very user-friendly. Everything we needed was in the box, and the sensitivity was good enough to detect isotypes even from supernatants with relatively low antibody concentrations. A must-have for any lab making monoclonal antibodies.
Q&As
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What specific information does this ELISA kit provide?
A: This kit determines the specific class and subclass (isotype) of mouse immunoglobulins present in a sample. It can distinguish between IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, etc. This information is crucial for selecting the correct purification method and secondary antibodies for your monoclonal antibodies.
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Is this kit suitable for screening hybridoma culture supernatants?
A: Yes, that is one of its primary applications. The kit is sensitive enough to detect isotypes directly from cell culture supernatants without the need for prior purification. This makes it an efficient tool for screening and characterizing new hybridoma clones in antibody development workflows.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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